• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Movement in and out of cells

Extracts from this document...

Introduction

Movement in and out of cells Helen Moth The cell membranes main function is to serve as a boundary between the cell and it's environment. It is just like other organelles in the cell in that it serves the cell by having it's own specialised jobs. In terms of beetroot the purple pigment is contained within the cell membrane, if this membrane is disrupted the pigment will be released. Temperature may be the cause of the disruption. High temperatures could distort the "active site" of the carrier, channel of gated proteins, therefore affecting the shape of the fluid mosaic model membrane which may release molecules that normally would not be allowed to be released (in our case the purple pigment), or allow other molecules in. Temperature basically disrupts facilitated diffusion. The picture above shows a close up view of a cell membrane, cell membranes are made up of fat molecules, or to be more specific phospholipid molecules with various large intrinsic and extrinsic, globular proteins suspended in them. ...read more.

Middle

So in theory a low temperature will not affect the permeability of the cell membrane and therefore no or little purple pigment will be able to diffuse and detected by the colorimeter. However as the temperature increases the cell membrane will become more and more permeable because the heat will distort the protein shape and the cell membrane will allow more pigment to escape. The aim of this experiment is to see the effect of temperature on cell membrane permeability, to help me get accurate results, am going to choose the temperatures- 0�C- no pigment should be released because at very cold temperatures the cell membrane phospholipid bi-layer and proteins should not be affected. Then after this every 20�, this will show me how the permeability of the membrane is effected as the temperature increases. I predict that as the temperature increases, the amount of pigment in the water will also increase. I expect that at about 35/40� the concentration of the pigment in the water will get to its maximum, then any higher temperature will be the same or similar to that at it's maximum temperature. ...read more.

Conclusion

Measure 15ml of distilled water into testubes, one for each piece of beetroot. 6. Remove the beetroot from current testubes, discard water and place into new testubes. Leave for 15mins to allow the pigment to diffuse. 7. Pour coloured water into colorimeter tubes put into colorimeter and record reading. PRACTICAL RESULTS Analysis My graph shows a general trend, that as the temperature increases the amount of pigment in the water increases too, however there are three anomalous results, these happen to be the three I had to do on a different day. This is because our lesson time is limited, so we had to finish the experiment in our own time. This is the cause of the anomolous results because by the time I came to finish the experiment, the beetroot was older than before, this could have affected the permeability before I even got to do the experiment. Also when I did the last results there was only enough beetroot to do two samples per temperature whereas before I did three per temperature, so when I took the averages, they would not have been as accurate as the results I took before. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Investigating the effect of temperature on the movement of pigment through beetroot cell membranes.

    4 star(s)

    Present the results in a table and plot a graph of colour density against temperature. HAZARD: HEATING WATER- WEAR SAFETY SPECTACLES. (My method for this preliminary experiment was written using information from a hand out sheet which gave me the volumes of water and timescales.)

  2. Marked by a teacher

    effect of temperature on beetroot pigment through membrane

    3 star(s)

    Remove the Co test tube. Wipe a sample test tube and place into the colorimeter, secure the cap and record the result. 10. Re-calibrate the colorimeter using the C1 test tube adjusting the percentage of transmission to 100% and repeat Step 9 for each sample test tube.

  1. Marked by a teacher

    An investigation to examine the effects of temperature on membrane stability in beetroot, by ...

    3 star(s)

    It converts 3,4-dihydroxyphenylalanine to betalamic acid. There are two things that can then happen to the betalamic acid; it can condense with amino acids/amines to form betaxanthin, or condence with clyco-COPA derivatives to form betacyanin. They are synthesized in the cytoplasm, then filtered out and stocked in vacuoles.

  2. Marked by a teacher

    The effect of temperature on the permeability of beetroot membrane

    3 star(s)

    o Remove the beetroot discs from the test tubes leaving the water solution in the test tube. o Repeat this experiment for all the other temperatures. * Select a suitable range for the independent variable or suggest conditions for the experimental treatment I will be carrying out this experiment at

  1. Marked by a teacher

    Effect Of Detergent Concentration On Membrane Permeability Of Beetroot Cells

    to a fair test, also when cutting to be sure to use the same ruler and making sure the cutting devices that is being use is washed before cutting the next 1 out of 4,1cm length beetroot. The beetroot should always be washed to remove the pigment leaked from damaged cells.

  2. TEMPERATURE ON BEETROOT PERMEABILITY

    The reason why 67�C is looking reliable and accurate than any of the other results may be because of the beetroot's membrane been already denatured. So even if the temperature is 65�C or 69�C, the same data of results for 67�C would still be derived.

  1. Membrane Permeability

    Molecules in a system at a higher temperature will have more energy and will move faster, and hence diffuse faster, than molecules of the same type in a low-temperature system. The size of the molecule also affects how rapidly it will diffuse.

  2. Investigating the effect of temperature on the movement of pigment through cell membranes.

    These three gradients show that the steepest part is section 2 and the flattest is section 3 which backs up what I have said. Section 1 - 25?C to 35?C Seeing as there wasn't that much pigment coming out of the cell, only a transmission of 56%, this indicates that the cell membrane is still intact.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work