• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

My aim is to design an experiment to compare quantitatively orange fruit, grapefruit and lemon fruit, with respect to the amount of glucose they contain. I will endeavour to make the experiment as fair as possible and aim to minimise the amount of errors.

Extracts from this document...

Introduction

Aim: My aim is to design an experiment to compare quantitatively orange fruit, grapefruit and lemon fruit, with respect to the amount of glucose they contain. I will endeavour to make the experiment as fair as possible and aim to minimise the amount of errors. I have been given 4% of glucose solution, benedict's solution and distilled water to find out the concentration of glucose of these three juices; orange, lemon, and grapefruit. The method of serial dilution has to be done to work out the concentration; this is because the concentration of glucose is far too concentrated. A dilution factor has to be worked out; I will be using a dilution factor of 5, which means the amount of glucose and the percentage of glucose will always divide by 2 as the dilution solution carries on. Theory Orange, lemon and grapefruit are all fruits which contain carbohydrates which include reducing sugars. Glucose is a hexose sugar, which is a monosaccharide with six carbon atoms in each of its molecules. Benedict's solution is a deep-blue alkaline solution used to test for the presence of the aldehyde functional group, CHO - which are present in reducing sugars. Benedict's solution changes colour when a reducing sugar is present because of a chemical reaction, which reduces the Copper II sulphate (which is soluble) ...read more.

Middle

The solutions should be labelled correctly and the lids of the solutions should are placed on the right bottles and should be secured tightly on The water bath is set at a very high temperature so caution must be taken to avoid any accidents from occurring. Removing and placing anything from the water bath must be taken very seriously and with great care, this will prevent any burns and scaling. Apparatus * 4% glucose solution * Colorimeter, * cuvettes, * Water bath, * Test tube rack, * Test tube, * Distilled water. * 10ml graduated pipette * 25 ml of Benedict's solution * Water Bath * Coloured pencil * Thermometer * Juices, apple, orange, grapefruit. Choice Of Apparatus Item What is it used for Reason for choice Colorimeter Instruments for the analysis of coloured solutions. Most accurate way for this procedure. Cuvettes Hold the solution, which will be placed in the colorimeter. 10ml graduated pipette An apparatus to remove and insert a solution. It is accurate Test tube To hold the solutions in. Most suitable apparatus to hold solutions Test tube rack Hold test tubes in place. Keeps the test tubes upright and organised 25 ml of Benedict's solution A biochemical test which shows the presence of a reducing sugar Test for glucose Distilled water Used to dilute the solution Water Bath 1000C Most accurate way to keep temperature desired the same. ...read more.

Conclusion

It is very important to read of the pipette as accurately as possible and to make sure that the reading is taken from the bottom of the meniscus. It will be necessary to make sure the equipment is clean to get concordant results. Some of the equipment will need to be washed out with a particular solution. For instance when washing out the cuvette you must wash it out with the solution that you will be using next, as if you washed it out with the distilled water this would effect the dilution concentration and therefore would affect the results. Table Of Results Apple Juice Test tube Glucose ml Distilled water ml Glucose % Colour Transmission Glucose Concentration 1 10 - 4 2 5 5 2 3 2.5 7.5 1 4 1.25 8.75 0.5 5 0.6 (0.625) 9.4 (9.375) 0.25 6 0.3 (0.3125) 9.7 (9.6875) 0.125 Grapefruit Juice Test tube Glucose ml Distilled water ml Glucose % Colour Transmission Glucose Concentration 1 10 - 4 2 5 5 2 3 2.5 7.5 1 4 1.25 8.75 0.5 5 0.6 (0.625) 9.4 (9.375) 0.25 6 0.3 (0.3125) 9.7 (9.6875) 0.125 Orange Juice Test tube Glucose ml Distilled water ml Glucose % Colour Transmission Glucose Concentration 1 10 - 4 2 5 5 2 3 2.5 7.5 1 4 1.25 8.75 0.5 5 0.6 (0.625) 9.4 (9.375) 0.25 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Exchange, Transport & Reproduction section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Exchange, Transport & Reproduction essays

  1. Peer reviewed

    The comparison of antibacterial properties of herbal products and standard antibiotics

    5 star(s)

    One interesting anomalous result found was in penicillin against E.coli, it wasn't massively irregular, however the other 5 results either had an area of around 41 or 45 and this one was at 31, affecting the percentage error quite badly.

  2. To compare quantitatively the concentrations of glucose and other reducing sugars in samples of ...

    * Test tube rack - for safe storage of test tubes. 1. Measure 2 cm3 of 4% (by mass) glucose solution in a 1 cm3 syringe and add it to a clean test tube. 2. Take 1 cm3 of the 4% glucose solution from test tube 1 and add it to test tube 2.

  1. beetroot experiment

    Some are unsaturated fatty acids, so it bent and fit together loosely. The more unsaturated they are, the more fluid the membrane becomes. The lower the temperature is, the less fluid it is as well. We know that when we heat something, you give it energy.

  2. Design an experiment to investigate the effect of temperature on the movement of a ...

    Continued care to the wound is also important. Washing the area gently with soap and water daily without scrubbing is best as the wound heals. Avoid putting products such as hydrogen peroxide, alcohol, or iodine solutions in the wound. These only delay wound healing and do not do anything to prevent infection.

  1. The Effect of Increasing the Amount of Glucose on the Rising of Dough.

    This can be shown as a graph like this. However because we are limiting the time for the yeast cells to respire - 10 minutes, the rise of the dough may come to a point where it becomes constant. This is because the yeast cells cannot respire any quicker within

  2. Role of blood in the body and experiment. The aim of this experiment is ...

    it's a dilute solution of salts, glucose, amino acids, vitamins, urea, proteins and fats. * White blood cells - these are involved in the immune system they find and stop pathogens that enter the body. * Platelets - these are what give blood the ability to clot.

  1. You are required to plan a procedure that will allow you to compare quantitatively ...

    light being received on the sensor and therefore the absorbance reading will be low. After doing this on my known glucose solutions after Benedict's test, I can use these readings to plot a standard curve, which can be used to determine the percentage of glucose in the three juices from their values of absorbance.

  2. This experiment aims to investigate the effects of 4 different types of fish food ...

    those fed on TetraPrima � Fry fed on Natural Foods would grow no faster than those fed on Aquarian Flakes Based on Protein content of the fish food alone, I would assume that guppy growth rate would be in the following order; Freeze Dried Brine Shrimp > TetraPrima > Aquarian

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work