• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Objective: To investigate the effect of using different carbon source on the growth of yeast cells.

Extracts from this document...


NAME: FARAH NADZIRAH ROSLI CLASS: ALM 7 M 13 Title: Using Different Carbon and Source for Growth Objective: To investigate the effect of using different carbon source on the growth of yeast cells. Background summary: Growth depends upon both the type of the nutrients available and their concentration. Cells are largely made up of the four elements: carbon, hydrogen, oxygen and nitrogen with smaller, but significant, quantities of phosphorus and sulphur. Accounting as they do for 90% of the cell's dry mass, all six elements are essential for growth. Hence, like all living organisms, microorganisms require an energy source, a carbon source and a range of nutrients for metabolic and cell growth. Microorganisms are small, easily dispersed and quick to multiply given a suitable environment. They grow on a wide diversity of substrates making them ideal subjects for commercial application. The microorganisms need organic carbon source, such as carbohydrates, as most of them are heterotrophic. Carbohydrates act as a respiratory substrate of cells. Microorganisms also need a nitrogen source for synthesis of DNA, RNA, ATP, coenzymes and chlorophylls. Apart from that, each species has its own optimum conditions within which it grows best. Respiration involves a series of metabolic pathway, which is a series of enzyme-controlled chemical reaction, where the product of one reaction becomes the substrate for the following reaction. It consist of two different type: aerobic respiration and anaerobic respiration. ...read more.


The gas dissolves in water to produce carbonic acid: CO2 + H2O H2CO3 Carbonic acid in water dissociates producing H+ and CO32- ions: H2CO3 2H+ + CO32- H+ ion produced make the solution become acidic. Thus, sodium hydroxide of known concentration is used to neutralise the acid contained in a 25 ml of each sample: H+ + OH- H2O The higher the volume of sodium hydroxide needed to neutralise the solution, the higher the concentration of acid present in the solution. The higher the production of acid by yeast, the faster the rate of respiration. It is essential to note that yeast cells in distilled water produced little CO2 because they do not have a source of sugar. Apart from that, distilled water is also acidic in nature causing it to be able to undergo neutralization by sodium hydroxide. The relative rates of respiration are linked with the growth of the yeast. When the yeasts are able to utilise a certain carbon source very well, it is said to be in a suitable growing condition. The yeast cells will grow faster by multiplying its number over time. The increase number of yeasts results in more respiration to occur in the culture medium. This experiment can be further extended to find the relative growth rate of yeast in different sugars by removing a sample from the culture at regular intervals. ...read more.


It is essential to take a concordance value between the first and second titration for calculating the accurate average titre value. The first and the second titre must not differ from 0.20cm3. To ensure that this is achieve, the titration must be done carefully with the conical flask containing the sample is swirled continuously during the titration. This is to ensure that the sodium hydroxide solution and the acid in the sample react evenly. In this experiment, the other variables are kept constant. For example, the amount of nitrogen, phosphorus and sulphur sources, which are ammonium phosphate and ammonium sulphate, are the same for every sample. The samples are kept in an incubator with the same temperature, at 25�C, for an overnight. Each flask is plugged with cotton wool during the incubation so that no carbon dioxide can escape from the conical flask to the atmosphere. Besides that, the volume and the percentage of the sugar solutions used are the same for sucrose and glucose, as well as the concentration of sodium hydroxide used to titrate the sample. These are to ensure that the effect observed is only caused by the different carbon source. The solutions only have a minimal risk to us but a safety precaution must also be taken. Take care to rinse any yeast spillage from skin in case of possible irritation. Conclusions: The respiration rate of yeasts is faster when glucose is used as the carbon source compared to that of sucrose because yeasts can utilise glucose better than sucrose for respiration. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Energy, Respiration & the Environment section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Energy, Respiration & the Environment essays

  1. Marked by a teacher

    effect of temperature on the rate of respiration in yeast

    5 star(s)

    Dehydrogenase enzymes remove hydrogen and give them to molecules of 2 NAD which gives out 4 NAD+ as a product. Where as the net production of the process is 2 molecules. However when glycolysis goes anaerobically oxygen-deficient conditions, NADH gets converted back to NAD through anaerobic mechanisms, whether homolactic or alcoholic fermentation.

  2. Marked by a teacher

    Effect of Anaerobic Respiration On Yeast

    5 star(s)

    However if the temperature becomes too high or too low the reactions they carry out can be prevented. If the temperature is too low not many, if any, of the substrate molecules and enzymes collide with each other, meaning no reaction can take place or the rate of reaction is greatly reduced.

  1. Marked by a teacher

    An investigation into the effect of different sugars on respiration in yeast.

    5 star(s)

    It can also be synthesized in the liver and kidneys from intermediates by a process known as gluconeogenesis. Fructose www.hyperphysics.phy-astr.gsu.edu/.../ sugar.html The most common place that fructose is found is in honey together with sucrose and glucose. It is also found in the disaccharide sucrose alongside glucose.

  2. What effect does substrate have on respiration in yeast?

    Apparatus Use 400ml beaker To hold the yeast suspension. 250ml Beakers To hold coloured tap water and the inverted graduated cylinder 100ml Graduated cylinder To hold the coloured tap water 25ml Pipette and pipette filler To measure the yeast solution and to transfer it from the beaker to the conical

  1. An investigation into the effects of temperature on the rate of anaerobic respiration of ...

    10 3.7 10.9 13.5 15 4.7 14.1 18.5 20 5.6 17.7 21.9 25 6.2 20.9 24.7 30 7.3 23.1 27.6 35 8.1 26.2 29.5 40 9.4 27.9 31.6 45 9.8 29.7 33.2 50 10.7 30.9 34.2 My average results suggest that 25 minutes in the water bath at 20 �C,

  2. Investigate the effect of changing the sugar concentration on the rate of respiration of ...

    Thus the rate of reaction would increase, resulting in the yeast respiring more therefore giving off more carbon dioxide gas, measuring a larger volume in the measuring cylinder. To start off this experiment I feel that it is suitable to explain the main facts of this study.

  1. Investigate the effect of bile salt concentration on the digestion of milk by the ...

    The pH on the sensor should now read 7. 5. Take the probe out of the yellow buffer solution. Clean the probe with distilled water and dry. 6. Place the dry probe into the red pH 4 buffer solution. The pH on the sensor should now read 4.

  2. the effect of bile concentration on the activity of the enzyme lipase during the ...

    Therefore by increasing the concentration of the bile salts, more lipid molecules will be emulsified providing the lipase with a bigger surface area for it to work on. However if the concentration of the bile salts continues to increase the rate at which the rate of reaction is increasing will

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work