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Observing fine detail of biological material investigated with a Microscope.

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Introduction

Observing fine detail of biological material investigated with Microscope Cells are too small to be seen with the human eye, so we need to use microscopes to magnify them. We study simple features of cells with a light or compound microscope. The earliest simple microscope was merely a tube with a plate for the object at one end and, at the other, a lens which gave a magnification less than ten diameters -- ten times the actual size. About 1590, two Dutch spectacle makers, Zaccharias Janssen and his son Hans, while experimenting with several lenses in a tube, discovered that nearby objects appeared greatly enlarged. That was the forerunner of the compound microscope and of the telescope. In 1609, Galileo, father of modern physics and astronomy, heard of these early experiments, worked out the principles of lenses, and made a much better instrument with a focusing device. Eventually, the light microscope was used to magnify many different thing of the world such as droplets of water. In the droplets of water Leeuwenhoek discovered that the water was filled with many tiny organisms. ...read more.

Middle

It helped me with my real experiment because I knew what to do. o I set up all the apparatus on the table. o I cut a small piece of onionskin from the onion using a forceps and I placed it on the slide. Then I put a drop of iodine on top of the onionskin and then I left it four minutes so that the onionskin will absorb the iodine. o After four minutes I put the cover slip gently on top of the onionskin. Then I put a droplet of water on one side of the cover slip. It forced the iodine, which was not absorbed by the onion to come out, and I used a piece of dry paper to suck the iodine. o I placed the slide under the microscope carefully to look at the onion cell until I could see a clear image of the cells. I used all three lenses 1. X4, 2. X10 3.X40. I was drawing the images on a piece of paper. ...read more.

Conclusion

I compared my results with the picture taken from the Internet, I found no errors or anomalies in my results, which means that my experiment went well. My strength in this was that I could work really well with scientific equipment and obtain the best possible results. I didn't have many weaknesses in this experiment but there was one weakness, which I noticed was preparing the slides correctly for the experiment but it didn't really affect my results, that's why my result were accurate. To improve my results I could have better results if I had used a bigger magnification to obtain the results, also if I could have researched more on plant cells. If I had better equipment then I had for the experiment then it would give me more accurate results. Still I obtained best possible results in the time given. Overall my experiment was useful for me because I learned many things from it and it gave me more knowledge about the scientific work. From this experiment I learned how to observe biological material with a microscope and how to prepare the slides. ...read more.

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