• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Observing Mitosis. The purpose of this experiment is to prepare a slide of actively dividing plant tissue and to observe stages of the cell cycle in living tissue.

Extracts from this document...


Observing Mitosis The purpose of this experiment is to prepare a slide of actively dividing plant tissue and to observe stages of the cell cycle in living tissue. We had to consider the duration of mitosis in relation to the whole cell cycle. First of all got some hydrochloric acid and put it into a water bath at 60�C. Then we cut off some root tips from garlic, we used the tips because it's where mitosis still takes place - the meristem. ...read more.


After cooling down again and drying, we put the root tips onto a clean microscope slide and cut off the tip of it. We had to make sure it was the meristem and not other differentiated root tissue. Then we macerated the root tip, this breaks up all the cells and makes them one layer. If not sufficiently macerated the layer may not be thin enough to observe cell nuclei. After this we added a small drop of acetic orcein stain so that we could see the cells under the microscope. ...read more.


Some of the stages we could have seen were prophase, metaphase, anaphase and telophase. It was most likely to find cells in the interphase because this stage is much longer than other stages. Some of the safety aspects: Hazard Risk Steps Taken Hydrochloric Acid Damage on contact to eyes and skin Wear goggles and a lab coat Glassware Sharp edges may cause cuts Be careful and put broken glass in the bin Sharps Cause cuts Be careful, tidy them away after Hot Water Bath Burn yourself Be careful Acetic Alcohol Corrosive Wear goggles and a lab coat ?? ?? ?? ?? ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

3 star(s)

*** Outlines and explains the steps in obtaining root tip squash slides to observe mitosis, and some 'trouble shooting' information. Could be improved by:
1)The addition of some relevant background information regarding the stages of mitosis
2)A clear explanation of why the various steps are completed
3)Some data, preferably manipulated with clear calculations to show how length of time spent in cell cycle phases can be calculated
4)Solutions to the potential problems that can affect the quality of the slides

Marked by teacher kerry Jackson 24/03/2012

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Find out if enzymes work faster or slower at different temperatures.

    5 star(s)

    We then carried on with the next experiment. Graph Analysis At 0oc there is very little energy in the enzymes and the substrate. This means that they are traveling very slowly. If they are traveling slowly then the chances that the enzyme and substrate will meet up is very small. The lower the temperature, the smaller this chance.

  2. Marked by a teacher

    Explain the link between cancer and mitosis. Describe how the chances of cancer developing ...

    4 star(s)

    Viruses that cause cancer usually carry their own oncogenes, which can cause uncontrolled cell division of the host cells.

  1. Marked by a teacher

    Beetroot Experiment. The purpose of this experiment is to determine the effect of temperature ...

    4 star(s)

    Repeating this experiment twice will hopefully help to remove the errors/anomalies and variations in the experiment. Method The method was carried out as described in the NEC handout Apparatus As described in the NEC handout Risk assessment There are several risks associated with this experiment I have listed them below and ranked there probability and severity.

  2. Discuss the advantages and disadvantages of cell specialization.

    Protists, which are not able to grow larger without organ systems, become easy targets for predatory forms. However, with the increase in size of an organism comes an increase in the complexity of its form. Such organisms would then require complex nutrients in their diets such as vitamins, and thus are unable to live off simple carbon and nitrogen sources.

  1. An experiment to find of the isotonic point of root vegetables cells in contents ...

    Also will be used label the water and 1Molar sucrose solution beakers. This will prevent my results from becoming mixed up. Will also be used to label the pipettes. * Scalpel- This will be used to cut the root vegetable to the accurate and appropriate length, this will minimize the

  2. To find out how different concentrations of sucrose solution affect the incipient plasmolysis of ...

    The overall net movement of water molecules is out of the cells of the root vegetable therefore the mass and length will decrease until equilibrium is reached, this occurs when the water potential inside the root vegetable is equal to the water potential in the sucrose solution and there is

  1. Investigation of the effect of adding different concentrations of NaCl to an enzyme-substrate (amylase-starch) ...

    Factors, which affect the action of enzymes, include: 1. Temperature - enzymes are inactive at temperatures lower than the optimum, and denatured at temperatures above the optimum temperature. 2. PH - enzymes are denatured at extremes of pH, and all enzymes have an individual optimum pH. 3. Substrate concentration - activity of enzymes increases with increasing substrate to a

  2. Osmosis Coursework. Investigation to determine the water potential of apple tissue and carrot ...

    * Cork borers could slip during use (there may be a lot of juices from the plant tissues) to care should be taken while using them; furthermore, they should be used on a flat surface to reduce the risk of slips.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work