• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Observing Mitosis. The purpose of this experiment is to prepare a slide of actively dividing plant tissue and to observe stages of the cell cycle in living tissue.

Extracts from this document...

Introduction

Observing Mitosis The purpose of this experiment is to prepare a slide of actively dividing plant tissue and to observe stages of the cell cycle in living tissue. We had to consider the duration of mitosis in relation to the whole cell cycle. First of all got some hydrochloric acid and put it into a water bath at 60�C. Then we cut off some root tips from garlic, we used the tips because it's where mitosis still takes place - the meristem. ...read more.

Middle

After cooling down again and drying, we put the root tips onto a clean microscope slide and cut off the tip of it. We had to make sure it was the meristem and not other differentiated root tissue. Then we macerated the root tip, this breaks up all the cells and makes them one layer. If not sufficiently macerated the layer may not be thin enough to observe cell nuclei. After this we added a small drop of acetic orcein stain so that we could see the cells under the microscope. ...read more.

Conclusion

Some of the stages we could have seen were prophase, metaphase, anaphase and telophase. It was most likely to find cells in the interphase because this stage is much longer than other stages. Some of the safety aspects: Hazard Risk Steps Taken Hydrochloric Acid Damage on contact to eyes and skin Wear goggles and a lab coat Glassware Sharp edges may cause cuts Be careful and put broken glass in the bin Sharps Cause cuts Be careful, tidy them away after Hot Water Bath Burn yourself Be careful Acetic Alcohol Corrosive Wear goggles and a lab coat ?? ?? ?? ?? ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

3 star(s)

*** Outlines and explains the steps in obtaining root tip squash slides to observe mitosis, and some 'trouble shooting' information. Could be improved by:
1)The addition of some relevant background information regarding the stages of mitosis
2)A clear explanation of why the various steps are completed
3)Some data, preferably manipulated with clear calculations to show how length of time spent in cell cycle phases can be calculated
4)Solutions to the potential problems that can affect the quality of the slides

Marked by teacher kerry Jackson 24/03/2012

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Beetroot Experiment. The purpose of this experiment is to determine the effect of temperature ...

    4 star(s)

    To ensure that temperature is the sole factor in this temperature it is important that all segments are removed from the water at the same time (or as near to it). If one segment was allowed to incubate in the water for longer than the others, there is greater time for diffusion of the beetroot pigment into the water.

  2. Marked by a teacher

    Aim: To estimate the solute potential of a plant tissue.

    Plasmolyses is the shrinkage of volume of a cell. This is caused by the falling of water concentrations and ultimately results in the contraction of the membrane.

  1. To find out how different concentrations of sucrose solution affect the incipient plasmolysis of ...

    mass decreases and as the molarity decreases the percentage mass increases, to obtain the isotonic point for the different root vegetables I will use the line of best fit where the line crosses the x-axis from a positive percentage gain to a negative percentage gain.

  2. Discuss the advantages and disadvantages of cell specialization.

    Xylem vessels in plants have thickened walls, impregnated with lignin and serve to conduct water and mineral salts through the plant and to provide mechanical support. Formed from procambial strand cells on the inner side of the strand in the developing stem, the xylem vessels effectively become continuous tubes when all the transverse walls have been broken down.

  1. An experiment to find of the isotonic point of root vegetables cells in contents ...

    * Core Borer (size 4) - This will used to cut an extract cylinder shape from the root vegetable required for this experiment. The diameter of the core borer needs to be the same ensuring that the experiment is fair; this will also ensure that each root vegetable has the same surface area to volume ratio.

  2. Investigation of the effect of adding different concentrations of NaCl to an enzyme-substrate (amylase-starch) ...

    Sometimes a cofactor can be a metal ion such as (Zn2+), which would encourage the binding of the substrate to the enzyme or it may have an active role in the reaction in centre of the enzyme active site. However, other times a cofactor can be a complex non-protein organic molecule also known as a coenzyme.

  1. A Level Biology revision notes

    are non-essential (produced by body) * Fat soluble o Vitamin A: vision, growth, reproduction o Vitamin D: regulates calcium levels, bone formation o Vitamin E: antioxidant (prevent cancer, Alzheimer) o Vitamin K: blood clotting * Water soluble o Vitamin C: antioxidant, wound healing, synthesis of adrenaline, bone formation o Vitamin B12 and folic acid: cell division (low levels cause anaemia)

  2. Osmosis Coursework. Investigation to determine the water potential of apple tissue and carrot ...

    Controlling Variables: To manipulate the independent variable, I will concoct sucrose solutions of different concentrations (0.0mol.dm-3, 0.2mol.dm-3...). To measure the dependent variable, I will weigh each piece of plant tissues before and after being immersed into the sucrose solutions. Weighing will take place on a balance, accurate to 2 decimal places in grams.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work