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Onion cell and cheek cell IntroductionTo stain the cells so the parts of the cell such as the nucleus are marked out and then it can be viewed

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Introduction

Onion cell and cheek cell Introduction To stain the cells so the parts of the cell such as the nucleus are marked out and then it can be viewed and observed more clearly under a microscope. The cells are stained and prepared on to a slide and with a cover slip placed on top to be ready to observe under a microscope. Equipment * Microscopes * Lamps * White tile * Cotton bud * Dropper pipettes * Slides * Cover slips * Labels * Forceps * Piece of onion Reagents required * Methylene blue (1 in 100) * Iodine Should be available * Laboratory coats * Gloves * Safety glasses Safety Points When using a light microscope we use the light form a lamp to brighten the microscope. ...read more.

Middle

Method I positioned a cover slip on top of the epidermis, again making sure that it was kept flat. On one side of the cover slip I placed a drop of iodine solution, which was a suitable stain for the plant cells. I placed the cut edge of a piece of filter paper to the other side of the cover slip which would draw a small amount of stain beneath the cover slip. I placed the slide under the microscope carefully to look at the onion cell until I could see a clear image of the cells. Viewing with a microscope I looked at the slide through low power then followed by high. ...read more.

Conclusion

This obviously makes the parts of the cell harder to identify as the view is unclear. As different parts of the cell are stained different colours in the lab it also makes it easier to spot any changes. Conclusion From the results it is possible to identify the basic structures of the two different samples. It is clear from the two diagrams that both cells have the basic components of a cell. Evaluation This experiment was fair to me because we only needed to do once and no repeats. I could see the differences between the cheek cell and onion cell (see the drawing of the cells in the next page). However, it allowed me to see the nucleus. I could see dozens of cells through the microscope. ?? ?? ?? ?? Naz Mariwan Science Coursework - Unit 1 ...read more.

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