• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Pectinase investigation.

Extracts from this document...


Pectinase investigation Aim My aim is to see if change in the temperature of the enzyme Pectinase will change the volume of the apple juice produced from the apple pulp, And also to see if the enzyme or distilled water will produce more than the other. Background Thousands of chemical reactions take place in our cells every day. We need these reactions to take place quickly to keep us alive. Chemicals called enzymes make reactions happen at a much faster rate. Chemicals that speed up reactions are called catalysts. Enzymes work on substances called substrates. The reaction always has to take place on a part of the enzyme called the active site. Provided that the substrate concentration is maintained at a high level and other factors such as pH and temperature are kept constant the rate of reaction is proportional to the enzyme concentration. Invariably reactions are catalysed by enzyme concentrations, which are much lower than substrate concentrations. Thus as the enzyme concentration is increased so will the rate of the reaction. Apparatus List Diagram Prediction I think that the higher the temperature is, the more juice will be produced from the apple pulp. ...read more.


4. And I will now for the apple pull into the field filter, the next pot I will have to do with someone else's help, I will pour the enzyme and apple pulp in at the same time and whilst I do this someone has to start up the stop clock. 5. I will Check the measuring Cylinder at two minute intervals for ten minutes. 6. To complete the room temperature one follow all of the previous steps except number three . Fair Tests The only thing I have to consider is the amount of apple pulp, Pectinase and distilled water to make sure it is all the same. Safety During the whole experiment I will be wearing goggles and clearing the entire experiment area. Also I will be working near a tap so I can wash my skin or eyes if the enzyme does make contact Measurements and Accuracy Below is a list of measurements. Pectinase - 5cm 3 Distilled Water - 5cm3 Apple Pulp - 20 cm3 I have to syringes so I don't mix any Pectinase in to the distilled water and vice versa. ...read more.


Despite the few minor mistakes that occurred during the experiment, the results of the actual investigation are very reliable and fairly accurate which can even be used for further research. I would consider having some different variables like: instead of changing the temperature I would change the concentration or even the fruit itself and have a different range of fruits and find out which one gives out the most juice. It would be very interesting to see how pectinase reacts at high and low concentrations and also discover the optimum concentration at which the enzyme works at its best. From general knowledge we could say that at high concentration pectinase will be denatured whereas at low concentrations not enough kinetic energy will be provided so the enzyme will be moving very slowly hence not producing any juice. To prove whether or not this is true we can extent the investigation by repeating the same thing but instead of changing the temperature of pectinase we could change the concentration in which the experiment is carried out every time. Haider Al-Marayati ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

3 star(s)

This is a well structured report.
1. The background section is well written, although the sources of information should be referenced
2. There is a very small amount of data that is collected in this investigation
3. The conclusion should included data from the investigation to back up the claim

Marked by teacher Luke Smithen 17/09/2013

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Analysing the vitamin C content in different fruit juices

    5 star(s)

    Only then, the known amount of mass of powdered vitamin C is dissolved into 100ml of distilled water to produce vitamin C solutions of different concentrations. This step is to increase the accuracy of the concentration of the vitamin C solutions prepared.

  2. Marked by a teacher

    Investigating the effect of enzyme concentration on the activity of cellulase.

    5 star(s)

    This rate result is the figure that is plotted on the graph. The 0% concentration can not be plotted on the graph as the result has been recorded as infinity. Table to show the highest and lowest rate (1/T) for wall paper paste to pass through the syringe barrel when

  1. Marked by a teacher

    effect of concentration of copper sulphate on the action of amylase to break down ...

    4 star(s)

    0.786 0.738 0.683 0.648 0.591 0.544 0.457 0.45 0.428 0.409 0.392 0.377 0.364 4. Conclusion This table shows the critical value for the corresponding number of pairs, and is to 5% level of significant. I have 21 pairs of results, so I will look between n = 20 and n

  2. To investigate how temperature affects the concentration of vitamin C in orange juice (specifically ...

    I am taking this to mean that without any really dramatic changes in heat, no more vitamin C molecules can be broken down. In considering this information, I would agree with it and I believe my results to be correct, as they appear to make sense.

  1. To find out how different concentrations of sucrose solution affect the incipient plasmolysis of ...

    Overall by observing my graphs most of my results for the different root vegetable cylinders under 0.4M gained in mass and the root vegetable cylinders above 0.6M lost mass as expected in my prediction because due to osmosis the net movement of water molecules move down there concentration gradient from an area of high water potential (low solute potential)

  2. Type - 1 Hypersensitivity Reaction

    Again this suggests that the tissue was still alive because carbachol works on the acetylcholine receptors. Sensitised tissue 2, a contractile response was noted and recorded for 0.2 and 0.4ml of 10-4 M histamine. The contraction was slightly higher when the 0.4ml (larger amount)

  1. An Investigation Into The Digestion Of Milk By Trypsin.

    I decided to calculate an average rate of reaction to make the results more accurate, because in some cases one experiment is not enough as many factor, for instance human error might affect the result, so I felt it would be best to calculate an average rate of reaction.

  2. Investigating the effect of temperature on the activity of free and immobilised enzymes.

    * using the same type of milk, as different milk may have different amounts of lactase (concentration of substrate is a confounding variable), * using the same concentration of lactase in immobilized beads and free solution which was 20%, * recording the glucose reading on the Diabur strips as they take 2 minutes before the result can be determined.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work