Plan an investigation to determine which part of a pineapple fruit " stem, flesh or leaves " has the highest concentration of bromelain.

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GCE APPLIED SCIENCE

                                            Unit 4 Cells and Molecules

Title of Investigation:

Plan an investigation to determine which part of a pineapple fruit – stem, flesh or leaves – has the highest concentration of bromelain.

Risk Assessment:

 

Prediction:  

I predict that stem has more concentration of Bromelain because stem part is tougher than the fruit so that means that they must have a lot of enzyme stored inside. The leaves containing the chlorophyll attracts the sunlight to make photosynthesis which is then transported to other part through the stem so that means that more are stored in the stem and the Bromelain is tougher and heavier so that means that more juice so more Bromelain. Bromelain is also used in pharmaceuticals industry to make capsules extracted from the stem so this proves me that the stem has the more enzyme concentration than the others.

Factors that effect enzyme reaction are:

Temperature:

When two chemicals react, their particles have to collide one another with sufficient energy in order for the reaction to take place but they will only react if they have enough energy that means the temperature have to be high in order for a reaction to happen quickly. So by increasing the Temperature the particles will move faster and the rate of reaction will increase, if the Temperature is higher then they don’t need of sufficient energy because the Temperature is more likely to be greater than the amount of Activation energy needed for the particles to collide faster. So therefore if the Temperature is less the particles will collide more slowly and the time taken for a reaction to occur will decrease but there is an optimum temperature for the reaction to occur is the temperature is increased more than the optimum level than the enzyme denatures causing the hydrogen bonds in the tertiary structure to break permanently. By using the water bath at 50°C will keep the temperature constant so do the reaction.

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Concentration of substrate:

If the concentration of the substrate is low then the particles will float about so there will be less collision in the particles and they wouldn’t take so long to react that means there are less collision taking place which means the rate of reaction decreases however if there are more concentration of the substrate then there will be more collision taking place that means that the rate of reaction increases. However the volume of substrate should be constant.

PH of enzymes:

All enzymes have a pH whether it is alkali or ...

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