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Planning an experimental procedure to investigate the conditions that effects the rate of growth of the fungus yeast.

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Introduction

Planning an experimental procedure to investigate the conditions that effects the rate of growth of the fungus yeast. Yeast is a fungus and alongside enzymes plays a big part in the process of Anaerobic Respiration. Sugar/yeast ======> energy + carbon dioxide +alcohol Enzymes To measure the rate of reaction I will measure the amount of carbon dioxide produced within a certain time. This is the easiest product to measure and also the product to give the most accurate and reliable results. To measure energy or alcohol would need very expensive apparatus which isn't available and also wouldn't give such reliable results. Enzymes catalyse the rate of the growth of yeast so therefore we need to know what factors affect enzymes, they are: 1. ...read more.

Middle

High heats destroy enzymes, as all enzymes are proteins. 2. pH - Most enzymes work best at neutral conditions, though different enzymes each have their own optimum pH. 3. Concentration of enzymes - if there are more enzymes, there are more active sites therefore more collisions are made creating more reactions therefore making the whole process a lot quicker. 4. Concentration of substrate - the substrate is the substance that the enzyme acts on. As the concentration of substrate increases the rate of reaction will increase as there are more collisions with the active site therefore speeding up the whole reaction. My prediction for this investigation is that as the concentration of substrate is doubled the rate of reaction will be doubled. ...read more.

Conclusion

For the solution to fit into a test tube adequately I will use a total volume of 40 ml so the yeast must be kept at a constant of 20 ml as a change in the concentration of enzymes will in/decrease the rate of reaction. The substrate will range: 1. 1Molar substrate= 20ml substrate 2. 0.8Molar substrate = 16ml substrate, 4ml water 3. 0.6Molar substrate = 12ml substrate, 8ml water 4. 0.4Molar substrate = 8ml substrate, 12ml water 5. 0.2Molar substrate = 4ml substrate, 16ml water The temperature must be kept at a constant of 40 C as this is the optimum temperature for the growth of the yeast enzymes. So I will expect the rate of reaction of 0.4Molar substrate to be double of 0.2Molar substrate due to my prediction made earlier. ...read more.

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