The overall experiment could of been more and reliable accurate if further tests were done, like mentioned in the conclusion, there was a limiting amount of pH buffer solutions used. There was not equal amounts of basic pH compared to acidic pH so the graphs would prove that it is ambiguous as it is impossible to tell the optimum pH for either of the amylases. However, from the two tests that were done they results were reliable as they were very similar with each other but a little change in pH 5 and 6 for fungal amylase.
The points on my graph were joined using straight lines from point to point. This might be because there could have been a different affect on these amylases with pH. This might mean there might be a slight variation in the amylases or a fault in the experiment. Despite trying to control possible variables there are a few areas within the plan which could have lead to inaccuracy in the results.
From my graph, no anomalous points could be seen which could suggest the experiment was conducted very well and the amylases work like this in nature, or if more tests were to be done this could be seen whether there still are any anomalies or not, as it may be contradictory for only the two tests done.
However it is still impossible to tell whether this is true and accurate or not and in order to do this more pH buffer solutions would need to be carried out to plot more points on the graph.
I would of done a preliminary study to get a more understanding of the experiment such as looking at different concentrations of starch, different concentrations of amylase enzyme, leaving it at a range of times to work at, etc which would help in explaining in greater depth the effect of pH.
In future if I had more time more the experiment, I would of liked to of worked with even more ranges of pH e.g. pH 7.2, 6.5 etc, this would make the experiment more accurate as there would be more points to plot on the graph. I would also of like to work with a bigger range of fungal and bacterial amylases in subject to availability.
