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Rates of respiration in crest plants, which are grown in different soils with a different pH

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Caroline van Bommel 23-04-2002 Group 4: Chemistry Lab Title: Rates of respiration in crest plants, which are grown in different soils with a different pH Aim: To measure the rate of respiration in Crest seeds which were embedded in different soils. Hypothesis: I think that the seeds, which are embedded in the soil sample with the most neutral pH, will show to have the highest rate of respiration. Variables: Control- amount of water added, the amount of soil, the amount of Crest seeds, the temperature of the soil and the duration of the experiment Independent- the area where the soil sample is from. Dependant- the amount of O2 consumption. Materials: For part 1 of the experiment Glass filter 100 mL Erlenmeyer flask For part 2 of the experiment Erlenmeyer flask 250 mL 100 mL beaker 10g of soil (either forest, moss, or potting soil) ...read more.


3) These aluminium packages must be placed it in the oven at 200oC for 30 minutes (so that all the water and organic matter will be desiccated.) 4) I placed 10 g of the backed soil in 8 ml of water and I stirred it gently. 5) Then I placed 10 drops of universal indicator fluid in the solution. (It was hard to see the colour, so we...read the next step) 6) With the aid of a filter I filtered out all the non-dissolved soil components, I repeated this until I had a clear solution left. 7) Once u have obtained a clear solution you can measure the pH of it. Part 2 1) Collect materials 2) From each type of backed soil we took 10g sample and placed it in the Erlenmeyer flask. ...read more.


Time Treatment 1 (mm3) Treatment 2 (mm3) Treatment 3 (mm3) 0 0.00 0.00 0.00 3 0.01 0.01 0.00 6 0.02 0.01 0.00 9 0.02 0.01 0.02 12 0.02 0.01 0.04 15 0.03 0.02 0.05 (The reason why I didn't continue recording the rate of respiration is because about 15 seconds after I hit the 15-minute mark the bubble in the treatment 3 pipette popped and directly after that the bubble popped in treatment 2 and then about a couple of seconds after that the bubble in treatment 1 also popped.) Graph 1: The amount of O2 intake in a time span of 15 min Table 3: The amount of Crest seeds that had sprouted after 4 days. Treatment nr. Origin of soil % Of Crest seeds that had sprouted 1 Forest soil 20% sprouted 2 Soil from underneath a moss plant 25% sprouted 3 Regular potting soil 90% sprouted ...read more.

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