• Join over 1.2 million students every month
• Accelerate your learning by 29%
• Unlimited access from just £6.99 per month
Page
1. 1
1
2. 2
2
3. 3
3
4. 4
4

# Reducing sugar plan

Extracts from this document...

Introduction

Planning Exercise Introduction The aim of the experiment is to test three samples of solution of fresh orange, lemon and grapefruit juice, and distinguish the amount of reducing sugars each one contains and to compare glucose standards. The experiment is going to be conducted in a similar manger to the preliminary experiments in that I am going to add the same amount of glucose solution, the same amount of distilled water and benedict's solution and then place it all in bath water and begin taking results from there on. Prediction I predict that all three of the juices will contain reducing sugars that will reduce the copper ions in the benedict's solution. The reason for my prediction is that all three of the juices originate from a fruit and will therefore contain the sugar fructose. Fructose is a monosaccharide. Monosaccharides are single sugar unit's, they have the general formula, (CH2O)n, because they have a C=O bond in the molecule they can contribute an electron to the copper, and therefore they are reducing sugars, in the process they themselves are oxidised. Reducing sugar + Cu2+ - oxidised sugar + Cu+ This C=O bond is known as the anomeric carbon atom, sugars reduce benedict's solution when the anomeric carbon atom is made available to reduce the copper ions. ...read more.

Middle

Variables In order for it to be a fair test the measurements of everything in the experiment need to remain the same. The temperature at which the solutions should be heated at 75 degrees, and left in for exactly 9 minutes. These are all the factors that we can control, the variables will of course be the different sugars which we are testing for the amount of glucose. Although the sample in themselves will be different the amount should remain the same so that it will be a fair test. The reason why we conduct a fair test is so that the results we get at the end of it will be reliable and so that we will be able to analyse the results confidently and make accurate predictions for future experiments. From preliminary experiments, it is possible to rely on the factors that we will control and know that the volume of solution, the temperature, etc.. is suitable for the experiment we are planning to undergo. Whilst conducting an experiment it is important to consider the risks and safety precautions, for example to wear safety goggles, and a lab coat etc.. This is to ensure we always remain safe throughout the experiment. ...read more.

Conclusion

From these results you are able to draw a calibration curve, which could then be used to find out the concentrations of unknown solutions by simply reading of the values on the curve. Outline From looking at the preliminary experiments we are able to see that the results were of high-quality and therefore we can feel confident that the following experiment we are about to perform will be a success and give us an accurate set of results. Out of all three of the experiments done the final one was probably most accurate as a colorimeter was used which means we will also be using a colorimeter in the experiment to take place. From looking at the preliminary experiments I am certain that I will not change any of the variables, or the method. Therefore I will carry on as normal, as a result my prediction remains the same. I believe all of the solutions will contain reducing sugars, hence turn the solution a red colour. The solution will reduce the benedict's solution because it is a monosaccharide, which are single sugar units. Sugars are able to reduce a benedict's solution when the anomeric carbon atom is made available to reduce the copper ions in the solution. The best way to test my prediction now, is to carry out my experiment. ?? ?? ?? ?? Stephanie Subramaniam 12CJ6 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Exchange, Transport & Reproduction section.

## Found what you're looking for?

• Start learning 29% faster today
• 150,000+ documents available
• Just £6.99 a month

Not the one? Search for your essay title...
• Join over 1.2 million students every month
• Accelerate your learning by 29%
• Unlimited access from just £6.99 per month

# Related AS and A Level Exchange, Transport & Reproduction essays

1. ## Design an experiment to compare quantitatively orange fruit, grapefruit and lemon fruit, with respect ...

Orange juice should have a higher concentration of glucose, followed by Grapefruit then lemon. I have predicted this because of a source I have obtained which showed that orange has the greatest number of grams of monosaccharides. The juice sample The amount of monosaccharides in grams Orange 8.5grams Grapefruit 5.3g Lemon 1.6g (Manuel of nutrition by Mafa)

2. ## My aim is to design an experiment to compare quantitatively orange fruit, grapefruit and ...

The juice sample The amount of monosaccharides in grams Orange 8.5grams Grapefruit 5.3g Lemon 1.6g (Manuel of nutrition by Mafa) If there is a greater number of monosaccharides there would be a higher concentration of glucose present. Benedict's test can prove this as the more reducing sugar present the greater the amount and the darker the colour of the precipitate.

1. ## To compare quantitatively the concentrations of glucose and other reducing sugars in samples of ...

Put all of the test tubes in a water bath at 75�C for 10 minutes. This will allow the Benedict's to react as much as possible, resulting more accurate test concentration readings. heat all of the test solutions for an equal length of time in order to keep the test fair.

2. ## My aim is to design an experiment to compare quantitatively orange fruit, grapefruit and ...

* The cuvettes will be rinsed out after use with the next solution. * The amount of time that the test tubes will lay in the water bath will be constant. Factors and Variables: Variable Independent variable These are the controlled set of values.

1. ## You are required to plan a procedure that will allow you to compare quantitatively ...

light being received on the sensor and therefore the absorbance reading will be low. After doing this on my known glucose solutions after Benedict's test, I can use these readings to plot a standard curve, which can be used to determine the percentage of glucose in the three juices from their values of absorbance.

2. ## Why did the colour leak out of cooked beetroot?

Diffusion will continue until the substance spread evenly throughout the whole volume. Small uncharged particles diffuse across the cell membrane, passing between the lipid molecules as they move down the concentration gradient. Small molecules like oxygen and carbon dioxide can rapidly diffuse across the membrane.

1. ## The main task of this experiment is to find out the concentration/volume of glucose ...

Bunsen burners can tend to be more dangerous than a hot water bath. This is as there is a naked flame alight in the laboratory. The hot water bath has minimal risks and there is also less hassle in getting the right temperature of the water as it is done automatically.

2. ## The sensitivity of the Benedict's Test-Investigation

we can see that the graph has formed a smooth curve indicating as the concentration of the glucose increases so does the percentage transmission of the colour.

• Over 160,000 pieces
of student written work
• Annotated by
experienced teachers
• Ideas and feedback to