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Report comparing different growth media, aseptic techniques and laboratory safety with own experiences

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Introduction

´╗┐Part 1- As the practical work had been carried out in a Category 1 microbiology laboratory, the required aseptic technique was to abide by personal hygiene rules such as washing hands before and after carrying out the three experiments, avoiding eating and drinking within the laboratory and tying the hair back. Personal Protective Equipment (PPE) that is required, consists of a lab coat; gloves and eye protection is worn if necessary. Also, benches are wiped before and after handling specimen in order to make a cleaner environment. The wastes which have had contact with the specimen are autoclaved before further disposal, this is done to prevent infectious microbes from being released into the environment. In Category 2 laboratories, microbiologists deal with pathogenic and/or infectious microorganisms that tend to have a moderate hazard. Due to their potential to cause diseases in humans, they are dealt with great care to prevent injuries that can be through needle sticks and cuts. ...read more.

Middle

Category 3 laboratories are used in working with indigenous agents that are posed to cause serious diseases through inhalation of particles or droplets. Working with these agents are extremely dangerous therefore they are strictly controlled by government agencies. Solid-front wraparound gowns and coveralls produced from material such as respirators are essential PPE. The ventilation system provides directional and controlled air flow through bringing clean air into the lab that has not gone through circulation. When dealing with highly hazardous specimen, the process must be done in a biosafety cabinet. Overall, the aseptic technique which has been used in the experiments in a category 1 laboratory is not as efficient as the techniques used in category 2 & 3 laboratories because they both include dealing with high biohazard specimen which need to be handled delicately in order to prevent infection from spreading. Whereas, in a category 1 laboratory the agents that were being handled with, were low hazard and therefore the precaution that was taken was to a low standard. ...read more.

Conclusion

This culturing technique is especially used for the aeration of liquids which have volumes that are greater than 200 ml. Non-Sparged Bioreactors that are mechanically agitated are capable of supplying a sufficient amount of oxygen for liquid volumes of microbial fermentation maximum 3 litres. A disadvantage of it, is that the stirring speed required may be up to 600 rpm before the oxygen for the culture does not become limited. Oxygen enters the fermenter liquid through the above head-space of the bioreactor and the liquid surface is broken via agitation which occurs continuously to increase the surface area and enables oxygen transfer. Sparged Bioreactors provide effective oxygen transfer for volumes of liquid which are more than 3 litres. As bubbles are introduced with the fluid, the oxygen transfer area increases. Agitation enables to break up bubbles and further increase oxygen transfer. Agitation speeds are significantly lower in sparged fermenters to enable efficient aeration when compared to non-sparged fermenters. Overall, the two techniques used are more effective in obtaining a higher yield of microorganisms than the plating technique as oxygen is transferred efficiently in order to allow the growth of the desired microorganism. ...read more.

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