• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Separation of Amino Acids by Paper Chromatography

Extracts from this document...


Separation of Amino Acids by Paper Chromatography Date:13/5/2011 Result: Amino acids Distance travelled /cm Rf = Distance travelled by amino acid / Distance traveled by solvent by solvent by amino acid Gyleine 7.4 3.2 0.405 Tyrosine 7.4 2.7 0.365 Leucine 7.4 5.1 0.689 Aspartic acid 7.4 2.2 0.297 Unknown B 7.4 3.3 0.446 Discussion: The solute is colourless such as amino acid, it can be located by spraying the ninhydrin solution to form a coloured spot. The colour of the complex formed between ninhydrin and aspartic acid is deep blue while that of other four are purple. Assumptions 1. Assume that the small area with concentrated spot of amino acid should be applied to obtain a deeper colour of spots. ...read more.


Errors 1. The origin line should not marked by the ball pen, since the ink will dissolve in the developing solvent and the line fades gradually or even an additional spot can be observed. 2. Ninhydrin solution should not spray too much on paper since it will blur and spreads the coloured spots. It's because the solvent that are used to dissolve ninhydrin can also dissolve amino acids. 3. There are some secretion on our fingers, for example amino acids and grease. It may result in the unexpected colour zone appears on chromatographic paper after spraying ninhydrin solution. 4. The shape of chromatographic paper may not exactly be the square-shaped, so that one side of the paper may be lifted and the speed of the solvent travel in one side is faster. ...read more.


2. Wear the gloves are necessary during the experiment in order to prevent the grease or amino acids on our fingers will contaminate the chromatographic paper and affect the accuracy of the experiment. 3. Use the smoothest side to be the starting point to ensure the solvent travel in on average. 4. Repeat the step of placing a small amount of amino acids in paper more than 10 times to make sure the spots will be present for good visual observation when the paper is developed. Conclusion: The paper chromatography did show that the unknown B could be defined to aspartic acid and tyrosine. By the chromatographic paper, the unknown B contains both deep blue and purple colour, and it matches the deep blue of aspartic acid. And the shape of the unknown B is teeth-shape, which is same as the tyrosine. So, the unknown B is made of aspartic acid and tyrosine. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Physical Chemistry section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Physical Chemistry essays

  1. Objective To illustrate ...

    On the contrary, if a less polar solvent is used, the non-polar amino acid will travel faster and the slowest one should be the polar amino acid. 3.> Why is it so important to avoid touching the chromatographic paper with your fingers?

  2. Identification of amino acids by chromatography.

    acid dots at various intervals, which can then be identified by measuring the distance between the spot and the pencil line. The relevant front (Rf value) of each amino acid can then be calculated by using the following equation: Rf value = Distance moved by compound Distance moved by solvent

  1. Investigating the Rate of the Reaction between Bromide and Bromate Ions in Acid Solution

    In tables 4.3.3 and 4.3.1, there are two anomalous results that are concordant with each other, suggesting that there was the same problem with both experiments. These could have been caused by contamination of the burettes - there were two instances of burettes becoming contaminated by other solutions over the

  2. Identification of amino acids by using paper chromatography

    * The mixture solution was swirl to mix, and the beaker was covered with a watch glass and left to stand. * Making sure by handing the square of chromatography paper by the top edge and place it on a clean sheet of paper and draw the lines and labels

  1. Antacid Lab. Are name brand antacids better than generic brand antacids to neutralize ...

    This helped the pH of both antacids to have a pH over seven. However it read that the antacids were weak considering the pH was only a little over a pH of seven. It didn't make sense that the weak bases stood up to around 2000 drops of stomach acid.

  2. Analysis of amino acids by paper chromatography

    Origin1 X Origin2 Asp (for aspartic acid) Origin3 Leu (for leucine) Origin4 Pro ( for proline) Origin5 Asp (for Asparagine) Origin6 Ph (For Phenylalanine) The amino acid is the added in spots to their corresponding origins. This is done using a capillary tube.

  1. I predict that the juice will have at least 2 or more of the ...

    * Hold paper at edges * Place paper on two clean sheets of file paper * Only use pencil for markings on the paper Use a micropipette to spot the various substances on the chromatogram. They should be in the following order: Juice, Juice + Alcohol, Amino acid mix, Asp, Leu, Lys, Pro.

  2. Chemistry open book paper - Nuclear Fission and Fusion

    is converted to energy, according to Einstein's equation: E=mc2 For the below reaction to occur the particles need to form a high-density, super hot, ionized gas - plasma. 2H + 3H 4He + 1n Tritium is produced by using lithium in the reactor, where neutrons from the deuterium-tritium reaction in

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work