• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Separation of Amino Acids by Paper Chromatography

Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  • Essay length: 606 words
  • Submitted: 01/12/2011
Share this essay:
AS and A Level Physical Chemistry

The first 200 words of this essay...

Separation of Amino Acids by Paper Chromatography

Date:13/5/2011

Result:

Amino acids

Distance travelled /cm

Rf = Distance travelled by amino acid / Distance traveled by solvent

by solvent

by amino acid

Gyleine

7.4

3.2

0.405

Tyrosine

7.4

2.7

0.365

Leucine

7.4

5.1

0.689

Aspartic acid

7.4

2.2

0.297

Unknown B

7.4

3.3

0.446

Discussion:

The solute is colourless such as amino acid, it can be located by spraying the ninhydrin solution to form a coloured spot. The colour of the complex formed between ninhydrin and aspartic acid is deep blue while that of other four are purple.

Assumptions

1. Assume that the small area with concentrated spot of amino acid should be applied to obtain a deeper colour of spots.

2. Assume that the edges of chromatographic paper are not touch the sides of the beaker since the solution at edges will move faster due to capillary action. The spots will bend and Rf value is affected

3. Assume that the beaker is saturated with

Read more
The above preview is unformatted text

Found what you're looking for?

  • Start learning 29% faster today
  • Over 150,000 essays available
  • Just £6.99 a month

Marked by a teacher

This essay has been marked by one of our great teachers. You can read the full teachers notes when you download the essay.

Peer reviewed

This essay has been reviewed by one of our specialist student essay reviewing squad. Read the full review on the essay page.

Peer reviewed

This essay has been reviewed by one of our specialist student essay reviewing squad. Read the full review under the essay preview on this page.