Page
  1. 1
    1
  2. 2
    2
  3. 3
    3

Separation of Amino Acids by Paper Chromatography

Extracts from this essay...

Introduction

Separation of Amino Acids by Paper Chromatography Date:13/5/2011 Result: Amino acids Distance travelled /cm Rf = Distance travelled by amino acid / Distance traveled by solvent by solvent by amino acid Gyleine 7.4 3.2 0.405 Tyrosine 7.4 2.7 0.365 Leucine 7.4 5.1 0.689 Aspartic acid 7.4 2.2 0.297 Unknown B 7.4 3.3 0.446 Discussion: The solute is colourless such as amino acid, it can be located by spraying the ninhydrin solution to form a coloured spot. The colour of the complex formed between ninhydrin and aspartic acid is deep blue while that of other four are purple. Assumptions 1. Assume that the small area with concentrated spot of amino acid should be applied to obtain a deeper colour of spots.

Middle

Errors 1. The origin line should not marked by the ball pen, since the ink will dissolve in the developing solvent and the line fades gradually or even an additional spot can be observed. 2. Ninhydrin solution should not spray too much on paper since it will blur and spreads the coloured spots. It's because the solvent that are used to dissolve ninhydrin can also dissolve amino acids. 3. There are some secretion on our fingers, for example amino acids and grease. It may result in the unexpected colour zone appears on chromatographic paper after spraying ninhydrin solution. 4. The shape of chromatographic paper may not exactly be the square-shaped, so that one side of the paper may be lifted and the speed of the solvent travel in one side is faster.

Conclusion

2. Wear the gloves are necessary during the experiment in order to prevent the grease or amino acids on our fingers will contaminate the chromatographic paper and affect the accuracy of the experiment. 3. Use the smoothest side to be the starting point to ensure the solvent travel in on average. 4. Repeat the step of placing a small amount of amino acids in paper more than 10 times to make sure the spots will be present for good visual observation when the paper is developed. Conclusion: The paper chromatography did show that the unknown B could be defined to aspartic acid and tyrosine. By the chromatographic paper, the unknown B contains both deep blue and purple colour, and it matches the deep blue of aspartic acid. And the shape of the unknown B is teeth-shape, which is same as the tyrosine. So, the unknown B is made of aspartic acid and tyrosine.

The above preview is unformatted text

Found what you're looking for?

  • Start learning 29% faster today
  • Over 150,000 essays available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Over 180,000 student essays
  • Every subject and level covered
  • Thousands of essays marked by teachers

Related AS and A Level Physical Chemistry

  1. Investigating the Rate of the Reaction between Bromide and Bromate Ions in Acid Solution

    0.0001M phenol solution o Error on pipette: o Error on volumetric flask: o Total error: 0.04% Making up solution C o Error on 20cm3 pipette: o Error on 100cm3 pipette (x4, since was used 4 times to obtain 400cm3 of H2SO4): o Error on volumetric flask: o Total error: 0.94%

  2. Identification of amino acids by chromatography.

    On the chromatography paper, there is a blurred, dark purple area (approximately 3mm in diameter), 4cms from the origin. If I apply it to the Rf equation: Rf value = 4 17 I can calculate that the Rf value is O.235 (3d.p.).

  1. Identification of amino acids by using paper chromatography

    shown on the diagram below * By using a clean melting point tube was dip into the solution of mixed amino acids and then touched briefly on the paper * Using a fresh tubes each time step 4 was repeat with the three other solution of single amino acids *

  2. An Investigation using Chromatography to determine the different Amino Acids

    x1 Rf factor of x1 = 0.113 220mm = 0.113 25mm x1 = Lysine Calculation for the Rf factor of x2 Rf factor of x2 = 0.272 220mm = 0.272 60mm x2 = Serine Calculation for the Rf factor of x3 Rf factor of x3 = 0.440 220mm = 0.440

  1. Analysis of amino acids by paper chromatography

    This is done using a capillary tube. Try to keep the spots as small as possible. You may want to practice the spotting of a solution on paper. Practice with water and a paper towel (or a piece of filter paper). Do not waste the chromatographic paper for practicing.

  2. To determine which solvent system gives the best resolution of separation of the individual ...

    When I say distinct, this means that the spots should be at varying distances from the origin line, with the values for their distances being very different. Their values should not be too close and as such give a bad resolution.

  1. Antacid Lab. Are name brand antacids better than generic brand antacids to neutralize ...

    Evaluation The lab procedure and the design of my experiment did contain some errors that could have been avoided to gather further accurate results. The first trial run of the antacid lab, our problem was the antacid read a pH of seven which was not significant.

  2. Objective To illustrate ...

    On the contrary, if a less polar solvent is used, the non-polar amino acid will travel faster and the slowest one should be the polar amino acid. 3.> Why is it so important to avoid touching the chromatographic paper with your fingers?

  • Over 180,000 essays
    written by students
  • Annotated by
    experienced teachers
  • Ideas and feedback to write
    your own great essays

Marked by a teacher

This essay has been marked by one of our great teachers. You can read the full teachers notes when you download the essay.

Peer reviewed

This essay has been reviewed by one of our specialist student essay reviewing squad. Read the full review on the essay page.

Peer reviewed

This essay has been reviewed by one of our specialist student essay reviewing squad. Read the full review under the essay preview on this page.