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Show the effect of temperature on enzymes.

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Jade Grizzle Monday 24th November 2003 To show the effect of temperature on enzymes Plan To show the effect of temperature on enzymes. I intend to show this by using catalase as my enzyme, which will convert Hydrogen Peroxide to water and oxygen. I will do 6 different experiments each with at a higher temperature than the last from 10?c to 60?c to see what the optimum temperature is: Experiment Temperature Enzyme concentration Hydrogen Peroxide 1 0?c 100 % 3cm� 2 10?c 100 % 3cm� 3 20?c 100 % 3cm� 4 30?c 100 % 3cm� 5 40?c 100% 3cm� 6 60?c 100 % 3cm� I will set up the water bath for all six different temperatures and then I will fill a 1000cm � beaker with water and a 25cm � measuring cylinder with water in to place upright in the water filled beaker (this is how I will be able to measure the volume). I will then take the delivery tube and place one end under the lip of the measuring cylinder and the other end with the bung onto the boiling tube. ...read more.


Therefore as long as there is plenty of substrate available the rate of reaction will increase linearly with enzyme concentration. The amount of volume of oxygen released and collected will always be higher at the beginning of the experiment and then will gradually slow down and eventually stop. This is because when the enzyme and substrate are first mixed, the amount of substrate is at its highest and wont be hard for enzymes to find a substrate to bind with its active site, but as time goes on its harder for an enzyme to find a substrate to bind with so the reaction slows down. Evaluation The suitability of the experiment was very good as it provided me with the required information and also the experiment was easy to carry out. This was because I only had to combine the hydrogen Peroxide and catalase together and place in the boiling tube with the bun on top. The oxygen that was released would travel up the delivery tube and up through the water filled measuring cylinder. This would force the water out of the cylinder, showing how much oxygen was being released every 30seconds. This allowed me to read off the amount released very efficiently. ...read more.


Therefore I could read the measurements off quite easy, but for enzyme concentration of 40% and 20% less oxygen was being released so I should of used a 10cm� measuring cylinder as the readings would have been more accurate. The glass syringes I used weren't accurate because as you extracted the Hydrogen Peroxide or the catalase from their beakers to the boiling tube the solutions would leak out from the sides resulting in less of the solution being used than required. This was inefficient because it meant there was either less substrate or enzyme concentration for the reaction and also the reaction would have been a lot slower than expected. I could of used pipettes instead of syringes as they would have been a lot easier to use and more efficient. As instructed I should have repeated each experiment 3 times so that if they were slightly inconsistent, when I worked out the averages they would have been more precise and would have followed the trend of the class results, this was however because I didn't have enough time to repeat the experiments for them to be more accurate. The class results followed the trend because averages were worked out from everyone's results and then placed on a graph, so any anomalous results wouldn't show up as much as they would of. ...read more.

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