• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  4. 4
    4
  5. 5
    5
  6. 6
    6
  7. 7
    7
  8. 8
    8
  9. 9
    9
  10. 10
    10
  11. 11
    11

TEMPERATURE ON CATALASE

Extracts from this document...

Introduction

TEMPERATURE ON CATALASE The effect of temperature on catalase Planning In this experiment, I will be investigating the effect that temperature has on the enzyme catalase. My hypothesis is that as the temperature increases, the rate of the reaction will increase up to a certain point after which the enzyme will denature. I predict that the denaturing point will be at about 50�C. This is the shape of the graph I expect to get for the rate of reaction: I made my hypothesis based on the knowledge I already have about enzymes. At lower temperatures, molecules are moving very slowly. Due to this, substrate molecules are not binding with the enzyme molecules, as the substrate molecules are moving too slow to collide frequently with the active site. As the temperature rises the movement of the molecules increases, causing collisions to happen more often. The speed at which the molecules are moving means they will collide with more energy, making it easier for bonds to be broken so the reaction can occur. Once the temperature increases past a certain point, the bonds that make the enzyme molecules structure begin to break, which deforms its shape and activity. At this point, the enzyme has now denatured. Apparatus I will be using Apparatus Use Water bath To keep the temperature precise Boiling tubes To hold the enzyme and substrate Thermometer To check the temperature is precise ...read more.

Middle

Time (s) Trial 1 Trial 2 Average 10 4 3 4 20 6 7 7 30 8 12 10 40 11 17 14 50 15 22 19 60 21 27 24 70 27 32 30 80 33 36 35 90 38 40 39 100 43 45 44 110 48 49 49 120 54 53 54 130 59 57 58 140 65 62 64 150 69 66 68 160 74 70 72 170 78 73 76 180 83 78 81 190 86 81 84 200 90 85 88 210 92 88 90 220 96 92 94 230 100 94 97 240 98 98 250 100 100 30�C Volume of oxygen collected (cm3) Time (s) Trial 1 Trial 2 Average 10 5 4 5 20 11 7 9 30 17 11 14 40 23 14 19 50 27 18 23 60 32 24 28 70 40 32 36 80 46 37 42 90 53 43 48 100 60 50 55 110 66 56 61 120 71 61 66 130 78 67 72 140 84 73 79 150 88 77 83 160 94 82 88 170 100 87 94 180 91 96 190 95 98 200 100 100 40�C Volume of oxygen collected (cm3) Time (s) Trial 1 Trial 2 Average 10 3 13 8 20 7 18 13 30 14 21 18 40 23 26 25 50 30 34 32 60 37 41 39 70 43 ...read more.

Conclusion

As the temperature increases, the rate of reaction increases until it reaches 50�C, where it decreases rapidly. The cause of this is most likely to be that somewhere between 40�C and 50�C, the enzyme began to denature. The optimum temperature on the graph is 40�C. *= segments marked in red on graph Evaluating evidence and procedures To make the experiment more precise, I carried out the experiment twice so I could use the results to work out an average. On doing this, on Graph 1, I noticed that the lines for 10�C and 50�C had slightly irregular shapes compared to the other lines. A number of things could have caused this, such as: Next time I do the experiment, I will carry out the method three times rather than twice. This will give me a better average to work from, making my results. I intend to make sure my apparatus has been sterilized and avoid sharing them, so as not to contaminate the contents. I will also use a wider range of temperatures, so as to find the exact temperature at which catalase denatures. Also, whilst carrying out the experiment, I'm going to keep the test tube contain the enzyme in the water bath at all times because, before, I removed the test tube from the water bath. The enzyme being in the air may have caused the temperature to increase/decrease from the temperature it was supposed to be at, hence having a big effect on the final results. ?? ?? ?? ?? ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work