Testing the heart rate of Daphnia with concentrated caffeine

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Biology                9th October 2007

Testing the heart rate of Daphnia’s using different caffeine concentrations

Hypothesis:-        The Daphnia’s heart rate will increase as caffeine concentration increases.

Equipment:-        Cavity Stick,

        Cotton Wool,

        Tally Counter,

        Microscope – Low power (x40) objectives,

        Stopwatch,

        Daphnia,

        Pipette,

        Beaker,

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        Water.

Controlled:-        Timing/Exposure to caffeine,

Variables        Timing of Daphnia under the microscope,

        Timing of Measurement.

Repetition:-        Each test uses a different Daphnia, a minimum of 5 tests must be done for each caffeine concentration.

How Are:-        Range of Caffeine Concentration,

Independent         Caffeine Tablets,

Variables         Equipment to measure volume of water,

Changed?

Safety Issues:-        Careful when transporting:

  • Daphnia,
  • Slides,
  • Microscopes.

Ethnical Issues:-        Keep Daphnia in the Caffeine as long as instructed, and then transfer Daphnia back into recovery (water).



Analysis:-        In this experiment ...

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