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The aim of this coursework is to compare the similarities and differences scientifically between the cheek cell experiment carried out in the labs of our school and the breast cancer pathology lab.

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Introduction

THE AIM The aim of this coursework is to compare the similarities and differences scientifically between the cheek cell experiment carried out in the labs of our school and the breast cancer pathology lab. Examples of the thing we have been asked to compare are thing like the method, procedures and equipment. To observe what they do we went to the Hedley Atkins/ ICRF Breast Pathology Laboratory at Guys Hospital located near Tower Bridge. At this the labs of this hospital they do diagnosis, prognosis on breast tissue and do the treatment, if necessary. Diagnosis means finding out what type of breast disease the patient has got. And to do this research the surgeon will have to carry out a biopsy on the breast tissue from the cancerous lump on the breast. The lab we visited receives three different types of biopsy from the surgeon. These biopsies are: 1. Excision Biopsy: The entire lump on the breast is cut and removed. 2. Incision Biopsy: A small piece from the lump is cut out and the rest is left on the patient. 3. Needle Core Biopsy: A small part of the lump is removed with a hollow needle and the patient's tissue inside is ejected after the needle is removed. These biopsies are very organised and not muddled up. ...read more.

Middle

6. The smear is observed under a low magnification of a microscope. Then when the cells are in focus maximum magnification is used to observe the cells with the best resolution. 7. After observing, immerse the slide and cover slip in a beaker of disinfectant. 8. The cotton buds are disposed in a polythene bags by the lab technician according to the local regulations. 9. Slides and cover slips are washed thoroughly, dried and re-used. COMPARING SIMILARITIES AND DIFFERENCES Similarities * Research: Both labs research on animal cells, which was from living tissues. * Stains: In both cheek cell experiment and breast cancer pathology, dyes are use to stain the cells so the parts of the cell such as the nucleus are marked out and then it can be viewed and observed more clearly under a microscope. * Preparation of cells: In both the experiments the cells are stained and prepared on to a slide and with a cover slip placed on top to be ready to observe under a microscope. * Microscopes: In both the laboratories we have seen the use of microscopes to observe the cells. Differences * Research: In the experiment carried out in our school lab we research or just observe our cheek cell but the hospital's laboratory researches on cells from the breast tissue and with a reason which is to look for malignant cells. ...read more.

Conclusion

* Storing the Cells: The cells that are researched in the lab are put in liquid nitrogen, by doing this the cells do not die, then the tissue is stored in wax, they cut this into slices and keeps one slice of the tissue in a tiny flat case for legal reasons, which is to keep a record of the patient because it may be needed in the future for things such as further treatment. In our lab we dispose the cells or wash it away as it is not part of a serious research. * Disposing the Cells and other equipment: After we finish the experiment we do not dispose the slides or any contaminated apparatuses but just wash it thoroughly removing all the cells and re-using the slides and the other equipment. In the lab it is considered very unsafe and unhygienic because used equipment will contain micro - organisms such bacteria and pathogens, therefore they keep the cells that they have researched stored, on the slides they used and store it as a record and dispose the apparatuses they used by incinerating or burning them, so all the germs are burned, killed and destroyed with all the used apparatuses, living no chance of contamination of the research. Lab Comparison Science Coursework 1 Argha Sarkar 10E ...read more.

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