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The aim of this experiment is to find out how temperature effects the action of the enzymes.

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Introduction

Enzymes Investigation Aim The aim of this experiment is to find out how temperature effects the action of the enzymes. Prediction I predict that as I increase the temperature in intervals of 10 it will increase the rate reaction of the enzymes, which will increase the products produced, so more oxygen will be given off. The reason why I predict this is because of the collision theory. The collision theory states that: Chemical reactions occur when particles of the reactants collide. They must collide with a certain minimum energy, called the activation center; otherwise no reaction will take place. If low temperatures is provided to the particles (substrates) it will then only cause few of the particles to receive the minimum activation energy so they will be less collisions so they will have less end products In our substrate at low temperature very few enzymes and substrates will be join together so less products is produced because less collisions. When we increase the temperature more particles will have receive more of the activation energy so it will cause more collisions. More of the enzymes and substrate will have received the minimum activation energy so will increase the collision so will increase the number of products, so as we increase the temperature it will cause more collisions so will cause more products produced. I predict that at the optimum temperature, which for more enzymes is between 40 - 50 it will reach the maximum reaction rate. ...read more.

Middle

Doing the preliminary I found that 0.25 grams of liver would be enough to react with the hydrogen peroxide and give off enough oxygen gas. The shape that I would use would be a long thing rectangular shape. It is important that they are the same shape, as surface area will affect the results so to keep it a fair test I will use the liver with similar weights and sizes. The reason why I have not placed the liver and the hydrogen peroxide in the same boiling tube straight away is because if I did this the catalase would start to break down the hydrogen peroxide before it reached the temperature that I wanted it to reach, this would them cause inaccurate results. When I place the liver and the hydrogen peroxide in the same boiling tube I will collect the oxygen gas that is given off. As when catalase reacts with hydrogen peroxide oxygen and water is given off. The reason I am collecting the oxygen gas is because it will show me how well the enzyme is working when the catalyse is put with hydrogen peroxide. There were three was that we could collect the gas and we did preliminary work to find out which method would be the best. One of the methods we tried was to use the gas syringe. We tested this by placing a delivery tube on top of the boiling tube, which had the liver and the hydrogen peroxide in it. ...read more.

Conclusion

The Bunsen burner was used instead of a kettle as with a Bunsen burner we could monitor the temperature and steadily heat the water to the temperature needed. * Liver- this contains catalase enzymes * Hydrogen peroxide- used as the substrate * Boiling tube/Beakers - hold the hydrogen peroxide and water baths * Stop watch- Time the reaction * Knife and white tile- to cut the liver * Weigh scale- to weigh the liver In the experiment we used 0.25 grams of the liver and 5 ml of hydrogen peroxide. We left the reaction for 3 minutes. We changed the time instead of 1 minute we did 3 minutes and the reason for this was because we felt that not enough oxygen was given off in this time and the oxygen displaced not a lot of water. We changed the time to 3 minutes as more water was displaced by the oxygen given off, better readings. Results 1st set of results 10 20 30 40 50 60 Start (ml) 15 11 26 21 17 22 End (ml) 17 16 32 26 22 27 Volume of oxygen (ml) 2 5 6 5 5 6 2nd set of result 10 20 30 40 50 60 Start (ml) 25 8 17 12 21 22 End (ml) 27 12 21 18 30 26 Volume of oxygen (ml) 22 4 4 6 7 4 Averages Temperature 1st (ml) 2nd (ml) Average volume added (ml) 10 2 2 2 20 5 4 4.5 30 6 4 5 40 5 6 5.5 50 5 7 6 60 5 4 4.5 1 ...read more.

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