- One potato (preferably large)
- 15 potato cylinders (4cm)
- Glucose solution
(Distilled water, 0.2m, 0.4m, 0.6m, 1m) 7ml of each
- 15 test tubes
- 2 test tube racks
- Sharp knife
- Timer (3600 secs)
- Cutting tile
- Measuring cylinder
- Cork Borer (0.4cm diameter)
- Masking tape
- Pen (for labeling)
- Ruler
- Electrical weighing scale
- Knife
- Masking tape (for labeling)
- Beaker
- Paper towels
Diagram:
Preliminary:
My reason for doing a preliminary is so that I can investigate which length would be suitable for my finale in order to get the best results. I can also find out the factors that might affect my results and rectify them in time for the finale.
Fair Test:
In order to get the most accurate results possible I will have to conduct my experiment under fair conditions. I will to keep certain things as constants and vary others.
- Size of the potato – I would have to keep this the same because the rate of osmosis can be affected by the size
- Keep the surrounding temperatures constant – The potato cells can be killed if the temperature is too high
- Use the same potato – different potatoes having different amounts of nutrients and water on the water
- Use the same amount of solution – If the solution is not completely covering the potato cylinders the uncovered parts will not be part of the reaction
- Weighing the potato – I have to try and weigh the potatoes as quickly as possible if not hey will remain in the solution for too long
- Dry the potatoes in exactly the way so that they will contain the same amount of water
- Keep the storage conditions of the test tubes all the same
Variable(s):
Concentration of solutions
(Distilled water)
(0.2m)
(0.4m)
(0.6m)
(1m)
Constant(s):
Size of potato slices
Time left in solution
Safety:
Safety is of the uttermost importance. I must make sure I am safe as well as the others students working with me.
- I will be using a borer to make the potato cylinders. I must make sure it is kept safely in order to cause the minimum damage.
- I will be holding a very sharp knife in order to get my potato cylinders the same size. This can be very dangerous if care is not taken.
- The test tubes are very fragile and can break relatively easy. These also must be taken into consideration where safety is involved.
- Make sure the solution is not taken internally – If so then contact a physician immediately
- Remove anything that might be an obstruction – coats, bags, scarves, hats, books etc.
- Tie back hair – the risk of getting your hair involved in the chemical reaction is decreased when you tie it back.
Method:
Tie back any loose hair or clothing
Remove all bags and coats from the area you are working to minimize the obstruction
Gather apparatus (as shown above)
Label the test tubes using the masking tape
Use borer to cut potato into cylinders
Use the knife to make all the potato cylinders the same size
Weigh each potato piece and record weight
Measure 7ml of each solution
Fill the test tubes with the different concentrations of glucose solution.
Add potato pieces to solutions at the same time (fair test)
Start stopwatch
Leave for 40mins - 1 hour
After 40mins – 1 hour remove from solution
Weigh again and record weight
Work out difference
Work out the change in mass
This a prediction of how I think my results will look:
PRELIMINARY
Results: As a table
Results: As a bar graph
Conclusion:
My preliminary experiment was a success although the results were a bit puzzling. The weight of the potato cylinder in the distilled water and the 0.2m solution increased rather than decreasing as I had predicted. I’m not sure whether this was meant to happen so it was a good thing that I did a preliminary first.
There wasn’t anything happening in the test tube that was big enough for me to see so I can’t say I have sufficient observations to back my prediction.
After this I’m going to do the experiment twice more and then take the average of those results to make one final results.
Evaluation:
I made two mistakes that delayed my experiment. I forgot to weigh the potato slices before I put them in the solution and when I did put them in the solution I didn’t label the different test tubes saying which solution they were in. As a result of this I had to set up the preliminary for a second time. The time I used to do the preliminary could have been used for the finale.
On the whole, the purpose for doing a preliminary was achieved since I found out that there are certain things that I needed to change for the finale. I will consider all the things I mentioned in my evaluation in order to rectify my mistakes and get the most precise results as possible.
I believe that there is still quite a bit more for me to learn before I can come to a final conclusion.
FINALE
1st Results: As a table
1st Results: As a bar graph
2nd Results: As a table
2nd Results: As a bar graph
Average results in a table:
Average results in a bar graph:
Average results in line graph:
Difference between before and after: in a line graph
Difference (only) between before and after: in a line graph
Conclusion:
The results of my preliminary did not fall completely in line with my hypothesis. For example I predicted that as the concentration increased the weight of the potato piece would decrease. But for the distilled and 0.2m glucose solution the weight increased. However, I realized this was because there was more water in the glucose solution and so it left the solution and went into the potato making it weigh more than before. This proved that osmosis had taken place whereas in the other three solutions plasmolysis had taken place.
Both the results for my finale were similar and so I have the confidence that I have got accurate results. I have no regrets except continuously spilling the solutions all over the place and delaying my experiment.
I did not notice any patterns that influenced my results.
Evaluation:
I learnt that there are many factors that can influence osmosis including temperature, time and concentration as was demonstrated I my experiment.
On the whole, the purpose for doing a preliminary was achieved because I found out that there are certain things that I needed to change for the finale.
I found that doing the experiment the second time was very easy because I had done the preliminary firstly.
I has sufficient evidence to support my prediction and say that as there is more water molecules in one membrane it’s mass will decrease due to the diffusion of water molecules.
It would have been interesting to see how osmosis would occur in different membranes. I would have liked to try it out with a different vegetable like a carrot or cucumber for example.
I would have been able to observe whether here is any relation to the experiment that I just done.
Biology – Osmosis Mrs. Connew