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The aim of this investigation is to investigate the enzyme activity by using Catalase and Hydrogen Peroxide.

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Biology experiment of catalyst Aim The aim of this investigation is to investigate the enzyme activity by using Catalase and Hydrogen Peroxide. Variables The factors, which will have an affect on the enzyme activity, will be: 1. Temperature 2. Concentration of enzymes 3. pH The variable in which I will be using to investigate the enzyme activity is the temperature. The rest of the variables will be kept constant and only the temperatures will be changed. Background information Enzymes help to speed up the rates of a reaction but do not get used up themselves. They are often called Biological catalyst and they speed up the reactions in living organisms. All enzymes are made from protein and specific enzymes are used for specific reactions. Enzymes are re-useable and can be affected by the temperature, and its pH. Temperature and enzymes Enzymes happen to work best at an optimum temperature. At low temperatures, the enzymes may become inactive and slow down or not work. But if they get heated again, then they will work again. If the temperature gets too hot, then the enzymes will become denatured. pH and enzymes Enzymes work best at a pH of seven (neutral). ...read more.


on top 12. At the same time, start the stop watch and time the for 30 seconds and record down the amount of gas produced in that amount of time in, via looking at the gas syringe 13. Once done, wash all the equipment and repeat the same experiment again for another two times 14. After, do the exact same experiment again for the rest of the different temperatures and so on. Range The variable I have chosen for this investigation is the change of temperature. The amount of liver to be used for each experiment will be 0.2g and the amount of Hydrogen Peroxide will be 25ml. I will time how much gas will be collected in 30secs! Results Amount of gas collected ( cm3 ) Temperature ( �C ) 1st Exp. 2nd Exp. 3rd Exp. Average 30 18 13 20 17 35 27 30 26 28 40 34 39 35 36 45 36 36 41 38 50 31 28 34 31 55 24 20 22 22 60 13 15 16 15 Here are the results from all the experiments. I have repeated each experiment twice and therefore have done a total of three experiments for each. ...read more.


There are also enough results done to make it fair. I also have enough evidence in the results to support all my points. How to improve Method and Accuracy Since I have taken about two to three lessons to complete my experiments, to improve my accuracy of the results and the method, I could have carried out all the experiments on the same lesson / day. The reason for this is so that I will get to use the same liver and not two different ones (as each lesson is supplied with a new liver). If this were to be done, then the same Catalase would have been used meaning it would be fair and more accurate. I can also improve the method by some how managing to weigh the liver without needing to touch anything, as then it won't have the chance to stick to things losing more liver, losing more Catalase! Extension To extend my work on this investigation, I will probably extend the temperatures so that I could see whether the Catalase would totally denature, meaning the rate of the reaction would stop and therefore no gas given off. Apart from this, I cannot really think of any more extensions to investigate the enzyme activity using temperature as the factor. Biology Coursework Tom Eastman ...read more.

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