• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The aim of this investigation is to investigate the enzyme activity by using Catalase and Hydrogen Peroxide.

Extracts from this document...

Introduction

Biology experiment of catalyst Aim The aim of this investigation is to investigate the enzyme activity by using Catalase and Hydrogen Peroxide. Variables The factors, which will have an affect on the enzyme activity, will be: 1. Temperature 2. Concentration of enzymes 3. pH The variable in which I will be using to investigate the enzyme activity is the temperature. The rest of the variables will be kept constant and only the temperatures will be changed. Background information Enzymes help to speed up the rates of a reaction but do not get used up themselves. They are often called Biological catalyst and they speed up the reactions in living organisms. All enzymes are made from protein and specific enzymes are used for specific reactions. Enzymes are re-useable and can be affected by the temperature, and its pH. Temperature and enzymes Enzymes happen to work best at an optimum temperature. At low temperatures, the enzymes may become inactive and slow down or not work. But if they get heated again, then they will work again. If the temperature gets too hot, then the enzymes will become denatured. pH and enzymes Enzymes work best at a pH of seven (neutral). ...read more.

Middle

on top 12. At the same time, start the stop watch and time the for 30 seconds and record down the amount of gas produced in that amount of time in, via looking at the gas syringe 13. Once done, wash all the equipment and repeat the same experiment again for another two times 14. After, do the exact same experiment again for the rest of the different temperatures and so on. Range The variable I have chosen for this investigation is the change of temperature. The amount of liver to be used for each experiment will be 0.2g and the amount of Hydrogen Peroxide will be 25ml. I will time how much gas will be collected in 30secs! Results Amount of gas collected ( cm3 ) Temperature ( �C ) 1st Exp. 2nd Exp. 3rd Exp. Average 30 18 13 20 17 35 27 30 26 28 40 34 39 35 36 45 36 36 41 38 50 31 28 34 31 55 24 20 22 22 60 13 15 16 15 Here are the results from all the experiments. I have repeated each experiment twice and therefore have done a total of three experiments for each. ...read more.

Conclusion

There are also enough results done to make it fair. I also have enough evidence in the results to support all my points. How to improve Method and Accuracy Since I have taken about two to three lessons to complete my experiments, to improve my accuracy of the results and the method, I could have carried out all the experiments on the same lesson / day. The reason for this is so that I will get to use the same liver and not two different ones (as each lesson is supplied with a new liver). If this were to be done, then the same Catalase would have been used meaning it would be fair and more accurate. I can also improve the method by some how managing to weigh the liver without needing to touch anything, as then it won't have the chance to stick to things losing more liver, losing more Catalase! Extension To extend my work on this investigation, I will probably extend the temperatures so that I could see whether the Catalase would totally denature, meaning the rate of the reaction would stop and therefore no gas given off. Apart from this, I cannot really think of any more extensions to investigate the enzyme activity using temperature as the factor. Biology Coursework Tom Eastman ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    Needles In the containers where syringes are obtained, there needles lying around. If the container is not handled with care it is possible that one of the needles penetrates the skin, creating the risk of an infection. The container has to be handled with care, and in case of the

  2. Amylase Investigation

    Some enzymes lose their activity when frozen. The presence of inhibitors. Enzyme inhibitors are substances that alter the catalytic action of the enzyme and consequently slow down, or in some cases, stop catalysis. There are three common types of enzyme inhibition - competitive, non-competitive and substrate inhibition.

  1. Catalyse Investigation

    Prepare boiling tubes, each with 25 ml of hydrogen peroxide in them (only prepare as many as will be used on that day). Prepare solutions of 20%, 17.5 %, 15%, 12.5%, 10%, 7.5%, 5%, 2.5% and 0%. There should be three test tubes of each concentration for the repeats.

  2. Catalase investigation

    * Volume of substrate; this is similar to concentration. A large amount of substrate to react with means the enzymes produce a lot of product. A small amount of substrate to react with means the enzymes produce a small amount of product. * Volume of pH buffer solution; this has to be kept at the same amount as the

  1. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    It is important for no bubbles to be taken up when filling the syringe and that no bubbles are created when squeezing out the solution into the boiling tubes. This can be helped by applying pressure to the syringe valve slowly and with a consistent speed.

  2. Factors that Affect Catalase activity

    An enzyme reduces the activation energy. See graph below. 5Energy Against Reaction Path: When an enzyme binds with a substrate the available energy has a greater effect and the rate of catalysis increases. Catalase: 3 "Catalase is a common enzyme found in root vegetables, such as potatoes and celeriac.

  1. Biology Coursework Catalase

    measuring the volume of each trypsin and distilled water with different measuring syringe (to avoid contamination) and then adding both to the test tube simultaneously. This will bring about more accurate result and less % error. Apparatus: * Test tubes x6 * Syringe x2 * Trypsin enzyme 15cm3 * Casein substrate 5cm3 * Distilled water (room temperature)

  2. Reaction of Catalase and Hydrogen Peroxide

    The sequence is summarized in the figure below. Effect Of Temperature On Enzyme Action The temperature of a system is to some extent a measure of the kinetic energy of the molecules in the system. Thus the lower the temperature, the lower the kinetic energy of the system and likewise

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work