• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The Breakdown Of Hydrogen Peroxide Via the Catalase Enzyme In Rates Of Reaction.

Extracts from this document...


The Breakdown Of Hydrogen Peroxide Via the Catalase Enzyme In Rates Of Reaction Catalase is an enzyme found in a range of living cells and tissues. For my experiment, I shall use yeast as a source for this enzyme. The independent variable is the concentration of substrate (in this case hydrogen peroxide) where as the dependant variables are the amount of substrate and enzyme you put in for reaction, Also the time for each reaction, and temperature of the water container. Apparatus Plastic Container 10cm3 and 100cm3 measuring cylinder Stopwatch Calculator Range of 2H2O2 concentrations (beginning at 2vols and ending at 10vols) ...read more.


the yeast was poured into the conical flask and the bung shut the conical flask so that the oxygen produced would not escape and would go into the 100cm3 measuring cylinder. Once five minutes had passed, I wrote how much volume of oxygen was in the 100cm3 measuring cylinder. Once that result was recorded, the conical flask was washed out and the 10cm3 measuring cylinder was washed out, the experiment was repeated again, but this time, the concentration of the hydrogen peroxide would go up every 2 vols up to the 10 vols. ...read more.


Rate of O2 Produced (cm3 min -1) 0 0 2 4.113333 4 8.446154 6 13.12667 8 17.5 10 44.58 The reason why 0 was included was to give the graph a starting point (i.e. from 0 to 4.113333) There was a steady increase from 0 to 8 vols, then from 8 to 10 vols, the concentration gradient shot up, this could be because there was more substrate concentration for the enzyme to react with. Sources of error :( Not all the yeast is the same, so therefore there would be different amount/concentration of catalase. :( PH temperature, if that was controlled more, then the experiment would have had better results. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    Make sure that the delivery tube to the burette is lined up as the first gas bubbles start to flow through it. If gas escapes being collected in the burette then start this experiment again. 19. Exactly when thirty seconds has passed record how much gas has been collected.

  2. Reaction of Catalase and Hydrogen Peroxide

    Than I had used the cork borer and cut the whole cylindrical piece of potato. After that using the ruler I had measured the length of the potato and make it 4 cm by cutting it using a knife. Then I had filled trough and measuring cylinder with water.

  1. An Investigation on the Effect of Enzyme Concentration on rate of hydrogen peroxide breakdown.

    Controlling the variables To ensure that only the independent variable, the concentration of the celery extract, is varied during each experiment, the other variables will be kept constant. As a result, the dependent variable, the rate of reaction will only be affected by the concentration of enzyme and will not be altered due to any other factor.

  2. Investigating the Different Concentration of Catalase On the Breakdown of Hydrogen Peroxide.

    In the same way as a key fits into a lock precisely, in this hypothesis the enzyme is the lock and the substrate the key. The lock and the key together (enzyme-substrate) form a temporary structure called enzyme-substrate complex. Enzyme molecules have a particular shape like a lock and only a particular substrate (key)

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work