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# The Breakdown Of Hydrogen Peroxide Via the Catalase Enzyme In Rates Of Reaction.

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Introduction

The Breakdown Of Hydrogen Peroxide Via the Catalase Enzyme In Rates Of Reaction Catalase is an enzyme found in a range of living cells and tissues. For my experiment, I shall use yeast as a source for this enzyme. The independent variable is the concentration of substrate (in this case hydrogen peroxide) where as the dependant variables are the amount of substrate and enzyme you put in for reaction, Also the time for each reaction, and temperature of the water container. Apparatus Plastic Container 10cm3 and 100cm3 measuring cylinder Stopwatch Calculator Range of 2H2O2 concentrations (beginning at 2vols and ending at 10vols) ...read more.

Middle

the yeast was poured into the conical flask and the bung shut the conical flask so that the oxygen produced would not escape and would go into the 100cm3 measuring cylinder. Once five minutes had passed, I wrote how much volume of oxygen was in the 100cm3 measuring cylinder. Once that result was recorded, the conical flask was washed out and the 10cm3 measuring cylinder was washed out, the experiment was repeated again, but this time, the concentration of the hydrogen peroxide would go up every 2 vols up to the 10 vols. ...read more.

Conclusion

Rate of O2 Produced (cm3 min -1) 0 0 2 4.113333 4 8.446154 6 13.12667 8 17.5 10 44.58 The reason why 0 was included was to give the graph a starting point (i.e. from 0 to 4.113333) There was a steady increase from 0 to 8 vols, then from 8 to 10 vols, the concentration gradient shot up, this could be because there was more substrate concentration for the enzyme to react with. Sources of error :( Not all the yeast is the same, so therefore there would be different amount/concentration of catalase. :( PH temperature, if that was controlled more, then the experiment would have had better results. ...read more.

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In this instance, I believe a graph, such as the one shown below will be produced. This will be the case in this investigation, as there is a set volume of substrate which means that there will not always be an excess and after time the number of substrate molecules will diminish along with the rate of reaction.

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