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The breakdown of wastes in liver cells produces hydrogen peroxide which is poisonous. This must be removed if the cell is to remain unharmed.

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Introduction

THE ACTION OF AN ENZYME Introduction The breakdown of wastes in liver cells produces hydrogen peroxide which is poisonous. This must be removed if the cell is to remain unharmed. Method 1. Label the test tubes A, B, C, and D. label the staining dishes B, C, and D. 2. Put 5cm3 of hydrogen peroxide in each test tube. Be careful -hydrogen peroxide is dangerous. 3. Put two cubes of raw liver into another test tube and put this in the water bath (set at boiling) for 2 minutes. 4. Use forceps to remove the cubes from the test tube. Place one cube on the staining dish B. Place the second cube in the mortar. 5. Grind the boiled cube (add a little water if necessary). Put the paste on staining dish C (use a glass rod if necessary). ...read more.

Middle

When the enzyme catalase is added to hydrogen peroxide, oxygen gas is given off. Liver can be proven that it contains catalase with the following pieces of evidence: A) the height of the froth differs to what treatment it is given, the un-boiled liver has a higher level of froth which means the boiled liver has some catalase destroyed due to the heat. B)There would be no reaction if catalase wasn't present. The height of the froth in test tubes A and D is more or less the same with the raw solid cube having a slightly higher level of 10.5cm whereas the paste has 10.4cm. This suggests that the surface area has no affect on the catalase although the boiled results suggest otherwise. To collect the gas and measure the volume of the gas accurately you would use a delivery tube. ...read more.

Conclusion

Use food processor to convert cubes to paste in doing this you will have the same surface area. The reliability of the evidence: The evidence in this experiment is not very reliable e.g. not having the same size cube can make a lot of difference to the experiment, the test tube diameter being different widths will dramatically affect the height of the froth which will make the results faulty. The boiled liver results are anomalous because there should be no froth as all the enzymes are supposed to be denatured, obviously boiling for 2min is not enough. Extension material (more experiments): -Firm conclusion was not possible in this experiment. -Series of experiments to gain adequate conclusion. -Boiling for 2 minutes was not enough to denature the enzymes. -Make sure to denature enzymes - a series of experiments to find at what temperature the enzymes become de-natured. Experiments for both of the above could prove useful in gaining an adequate conclusion and a more accurate set of results. ...read more.

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