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The effect of alcohol on the stability of cell membranes-plan for an experiment.

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Transfer-Encoding: chunked Effect of alcohol on plant cell membranes-Introduction/Planning The AIM/PROBLEM The aim of this investigation was to investigate as well as analyze the effects different types of alcohol has on the stability of cell membranes (the membranes of potatoes and beetroot). RATIONALE This investigation carries a high level of importance as well as relevance to humans in general, in other words it relates to society and our lifestyles, in relation to alcohol. As proven continuously, scientifically (generally) as well as socially, alcohols are dangerous to us living organisms. But with this being said, if one was to look on a deeper level one will find that alcohol is highly dangerous to living organisms due to the damage they inflict on cellular membranes. This causing a chain reaction of damage, due to the fact that, damage to a cell membrane means the content within that cells is exposed and pours out of that cell, in tern killing the cell. With the use of the example being used within this investigation within the beetroot plants, the vacuole consists-along with other a water-soluble red pigment, known as betacyanin, which provides the beetroot its color. ...read more.


So for the section of which involves the beetroots, when the red-like pigment (from the beetroot) leeks into the alcohol/solution, the alcohol/solution will turn red. As stated previously the strength of the colour within the solution must be similar to the amount of cellular damage the beetroot has sustained. With this being said, as the concentration of pigment within the solution increases, the intensity of the the light of which is able to pass through the solution decreases. Equipment Chemical/liquid Alcohol Concentration (%) Methanol (50 ml of each concentration) 0 20 40 60 Ethanol (50 ml of each concentration) 0 20 40 60 1-Propanol (50 ml of each concentration) 0 20 40 60 Biological 5 Beetroots (canned or fresh) 5 Potato Glassware 54 test tubes 10 ML (glass) measuring cylinder Other equipment Gloves White tile Marker Forceps Marker Stop watch Test tube rack Balance Cork borers (6) Distilled water Scalpel Weighing boats Colorimeter Ruler Face mask HYPOTHESIS: -Potato It is uncertain to know the concrete possible outcome of how the weight will change for any of the concentrations being used, or if there will be a change at all. ...read more.


Forceps This was used in order to carry the pieces. Tissue This was to clean the work area, due to the fact that it got a bit messy at times. ABSTRACT In this investigation, the effect of various alcohols (at various concentrations) on the stability of the cell membrane of beetroot as well as potato is being investigated as well as analyzed. Methanol, Ethanol, as well as 1-Propanol are the chosen alcohols of which the beetroot as well as the potato pieces are going to be placed in. This is to be able to analyze their effect on the permeability of the cell membrane. For the beetroot this will be done with the use of two methods, the first way is to measure the change in weight of the pieces before being put in the alcohol and after. The second way is to measure the level of intensity of light of which is able to pass through the various solutions. For the potato, this will only be done using one method, which is to measure the change in weight of the pieces before being put in the alcohol and after its been put in alcohol (after a certain period of time). The results for both, the results are calculated and analyzed and then a conclusion is made. Reference http://www.ncbi.nlm.nih.gov/pubmed/3526990 http://www.westminster.edu/about/community/sim/documents/seffectofalcoholoncellmembranes.pdf ...read more.

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