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The effect of bile salts on the release of Anthocyanin from Beetroot cell membranes.

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Introduction

The Effect of Bile Salts on the Release of Anthocyanin from Beetroot Cell Membranes Analysis Results Table Bile Salt Concentration (g/100g) Average Absorbance (Arbitrary Units) Data set 1 Data set 2 Data set 3 Mean 0.0 0.02 0.04 0.08 0.05 0.1 0.08 0.05 0.11 0.08 0.2 0.15 0.13 0.11 0.13 0.3 0.21 0.24 0.22 0.22 0.4 0.28 0.30 0.24 0.27 0.5 0.40 0.35 0.26 0.34 0.6 0.31 0.27 0.28 0.29 0.7 0.36 0.34 0.33 0.34 0.8 0.43 0.35 0.33 0.37 0.9 0.46 0.38 0.35 0.40 1.0 0.50 0.45 0.39 0.45 Data figures in red are anomalous results Spearman Rank Statistical test I will use the mean values from the 3 complete data sets to find the Spearman rank correlation. Bile Salt Concentration (g/100g) Rank R1 Mean Average Absorbance (Arbitrary Units) Rank R2 Difference / D (R1 - R2) D2 0.0 1 0.05 1 0 0 0.1 2 0.08 2 0 0 0.2 3 0.13 3 0 0 0.3 4 0.22 4 0 0 0.4 5 0.27 5 0 0 0.5 6 0.34 7.5 1.5 2.25 0.6 7 0.29 6 1 1 0.7 ...read more.

Middle

The table, ignoring anomalous results, shows a trend of the absorbance of the solution to increase as bile salt concentration increases. The difference of mean absorbance (arbitrary units) between bile salt concentrations of 0.0g/100g and 1.0g/100g is 0.40. This is a significant change which cannot possibly be put down to coincidence. Each of the 3 data sets, ignoring anomalous results, shows the increase in average absorbance as the bile salt concentration increases. The differences of absorbance between bile salt concentrations of 0.0g/100g and 1.0g/100g vary between the three data sets, but all show a positive increase in absorbance as bile salt concentration is increased. The graphs show graphical representation of the results obtained. The positive correlation of the graphs show the general trend that absorbance increases as bile salt concentration increases. The absorbance has increased due to the increased release of anthocyanin from the vacuole and cell by diffusion down the concentration gradient. There is a scientific explanation of why this occurs. Anthocyanin is located in the vacuole of the beetroot cell as shown in the diagram below. ...read more.

Conclusion

Bile salts have a similar effect on the phospholipids in the membrane. Because the phospholipids consist of a hydrophobic tail and a hydrophilic head, the bile salts join with the individual phospholipids and effectively emulsify the phospholipids by turning them into liquid due to the large membrane turning into individual phospholipids. This will increase the release of anthocyanin from the tonoplast and cell membrane because there will a more permeable membrane because the bile salts will cause the emulsification of the phospholipids. The anthocyanin will therefore be able to diffuse out of the cell due to lower concentration of anthocyanin outside the cell, than inside the cell (down the concentration gradient) because there will be large spaces in the cell membrane due to the emulsification of some of the phospholipids in the membrane phospholipids bilayer. Increasing the bile salt concentration increases the number of bile salt molecules. A higher bile salt concentration will therefore have more bile salt molecules to make more punctures in the cell membrane, and so more anthocyanin will leak. Sanjay Devalia ...read more.

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