The effect of bile salts on the release of Anthocyanin from Beetroot cell membranes.

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Sanjay Devalia

The Effect of Bile Salts on the Release of

Anthocyanin from Beetroot Cell Membranes

Analysis

Results Table

Data figures in red are anomalous results

Spearman Rank Statistical test

I will use the mean values from the 3 complete data sets to find the Spearman rank correlation.

Σ D2 = 3.5

The experimental hypothesis that I have produced is that as the bile salt concentration, in which the beetroot discs are placed in, increases, the absorbance of the solution which is placed in the colorimeter also increases.  Therefore, there will be a positive correlation between bile salt concentration and absorbance of the solution.

The null hypothesis of this experiment is that there is no correlation of the results.  

rs (Spearman Rank Statistical test) = 1 -     6 Σ D2 

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                                                n(n2-1)

                                       

                                   

=  1 -      6 X 3.5                = 1  -       21                         

 11(112-1)                          1320

rs = 1 - 0.016

rs = 0.98

The critical value at 5% significance for 11 pairs of measurements is 0.62.  As you can see, the Spearman Rank value achieved is 0.98, which exceeds the critical value. Therefore, you can reject the null hypothesis because the value achieved exceeds the critical value at 5% significance for the number of data sets. The results therefore show a strong positive ...

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