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The Effect of Copper Sulphate On Catalase Activity.

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THE EFFECT OF COPPER SULPHATE ON CATALASE ACTIVITY SCIENTIFIC BACKGROUND. In each individual cell of a human there are many chemical reactions taking place, performing the necessary functions for being a large, complex, multicellular organism. Chemical reactions are catch-all phrase for the breaking and reforming of chemical bonds between molecules (substrate(s) of the reaction) which are transformed into different molecules (product(s) of the reaction). Chemical reactions can occur spontaneously (without added energy or intervention), and indeed some of the chemical reactions necessary for life processes can be spontaneous. But in the case of many processes in our body, these processes are actually many chemical reactions that occur in a specific order. For example, in order to get energy out of a molecule of glucose, a series of reactions must take place in order to break the bonds between the carbons of the glucose molecule. Plus, you have to rely on a series of chemical reactions that break down starch into glucose molecules in order to have glucose molecules in the first place. Now, if you had to rely on these reactions to take place spontaneously, you would wait a very long time. ...read more.


If the molecular structure is disrupted, the enzyme ceases to function as the active site no longer accommodates the substrate. The enzyme is denatured. To control this variable, the temperature was maintained at a fairly constant level that allowed the enzyme to work effectively (room temperature, approximately 23�C). > PH Any change in pH affects the ionic and hydrogen bonding in an enzyme and so alters it shape. Each enzyme has an optimum pH at which its active site best fits the substrate. Variation either side of pH results in denaturation of the enzyme and a slower rate of reaction. In this experiment, the pH was kept constant using a pH 72 (buffer), selected to maintain a pH level suited to the enzyme by being equal to the natural environment of the enzyme (yeast). Substrate Concentration When there is an excess of enzyme molecules, an increase in the substrate concentration, produces a corresponding increase in the rate of reaction. If there are sufficient substrate molecules to occupy all of the enzymes� active sites, the rate of reaction is unaffected by further increases in substrate concentration as the enzymes are unable to break down the greater quantity of substrate. ...read more.


I decided to collect as much gas as possible if I had time, because this would reduce the percentage errors. If the point on the gas syringe is not at eye level, there will be accuracy in my readings or results. > Stop the stop clock as soon as the gas syringe is full of gas (again, make sure that this point is at eye level to eliminate parallax error). The stop clock should be stopped the moment the oxygen collector reaches the edge of the gas syringe. > Enter this time and the amount of oxygen collected in, the correct column for which repetition of the experiment it is on the table. > Take out the beaker and rinse with distilled water before continuing the experiment. > Repeat the experiment three times for deferent concentration of hydrogen peroxide, and water. I am repeating these experiment three times because, the more repetitions the better, the result. Notes: First come the sheets of the table in which I recorded my results. Then the sheet on which I put the information to be transferred to graphs. Then come the graphs in a numbered order. They need no explanation as they have complete headings. The computer plotted the lines of best fit and some are more roughly sketched than others are. 1 BIOLOGY COURSE WORK BY OLABIMPE - YUSUFAROWOLO ...read more.

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