The Effect Of Enzyme Activity

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The Effect of Enzyme Concentration

On Enzyme Activity

Aim:

To find the effect of enzyme concentration on enzyme activity, by recording how various concentrations of enzymes alters activity. I will change concentrations by using a base solution to dilute the enzyme concentration. I then shall make a prediction and see whether I am correct or not after reviewing and analysing all my readings.

Hypothesis:

I predict, the greater the enzyme concentration, the greater the activity. The control (set at 0%) will have no activity - as my prediction suggests the greater the enzyme concentration the greater the enzyme activity. This is as the greater the concentration the greater cumulative surface area of active sites to react upon.

Apparatus:

* Beakers

* Pipette

* Standard acidified protease solution

* Gelatine jelly

* Standard laboratory glassware and equipment

* Stop clock
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* Ruler

* Thermometer

* Filter funnel

* Conical flask

* Glass rod

Method:

I started by ensuring that I filled out a risk assessment of possible fault or accidents during certain procedures of the practical. This was for example wearing goggles and gloves as beef gelatine jelly was being used, as well as a lot of glassware. After I had made certain that I had my workstation clear and that all potential hazards had been minimized or avoided, I began the practical. The first step of this was to collect the ...

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