• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The Effect of Enzyme Concentration on Enzyme Activity

Extracts from this document...


Amy Bowring The Effect of Enzyme Concentration on Enzyme Activity Aim: To investigate the effect of a change in enzyme concentration on activity of that enzyme. The Pancreas produces enzymes through the pancreatic duct to the small intestine which help digest our food. In people who have cystic fibrosis, the pancreatic duct frequently becomes blocked restricting these enzymes. The aim of this investigation is to investigate the effect of a reduction in enzyme concentration on the rate of reaction. In this case the breakdown of protein by protease enzymes. The independent variable will be a pepsin enzyme concentration which will decrease from 5% to 1%. This is how the enzyme concentration will be reduced. ...read more.


Hypothesis: I believe that as the concentration of enzyme decreases, the rate of reaction will also decrease. This is because there are fewer enzymes to react with the substrate. However if there is too much enzyme the rate will not necessarily continue to increase, it will plateau. This is because eventually all the substrate has enzyme to react with and anymore will have no effect as it has nothing to act on. Apparatus: * Funnel * Pipettes * 2 Stop Clocks * Test Tubes * Spatula * 2 150cm3 beaker * 75g of 2.0% gelatine solution (fully set) * pH 2.8 buffer * Pepsin solution (5%) * Glassware for diluting enzyme Method: * Suspend funnel over an empty beaker. ...read more.


77 132 99.2 2 118 155 245 37 126 241 153.7 1 94 241 336 32 332 207.0 Graphs: Conclusion: My results support my hypothesis that as the concentration of enzyme decreases, the rate of reaction will also decrease. My results appear fairly reliable and my experiment was repeated 6 times and an average was taken at the end. However each experiment was repeated by six different group so some of the controlled variables may have been controlled differently, eg, the way the gelatine jelly and enzyme are mixed. However this has not seemed to have effected the experiment too much. One problem I encountered was that the original funnel I used was too wide and the mixture just slipped right through each time. I simply corrected this my using a thinner funnel. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Investigating the effect of enzyme concentration on the activity of cellulase.

    5 star(s)

    mixed with the different concentrations of cellulase To draw the error bars on the graph, the rate has to worked out for the highest and lowest valves of the time taken for the different concentrations of cellulose/wall paper paste to pass through the syringe barrel.

  2. Marked by a teacher

    Factors effecting enzyme activity

    4 star(s)

    how quickly oxygen was produced and whether I would be able to measure it accurately, I decided that I could measure the amount of oxygen produced every 10 seconds for a minute, because after one minute most of the oxygen had been produced and it would be pointless to carry on after that.

  1. An Investigation Into the Effect of Substrate Concentration On the Rate of Enzyme Activity.

    7.5 7.5 10 10 10 12 20oC 11 11 12 12 12 14 ? R1 = 21 ? R2 = 57 U1 = n1 x n2 + 1/2 n2 (n2 + 1) - ? R2 U1 = 6 x 6 + 1/2 6 (6 + 1)

  2. Free essay

    Cystic Fibrosis

    Like the same way you can have your dad's nose or your mum's eyes. Cystic fibrosis is a genetic disease that can be very serious and fatal. The disease can affect most parts of your body. You can be either a carrier which you don't suffer from the disease but you can pass it on genetically (through offspring)

  1. Investigating the effect of pH on the activity of an enzyme.

    * Leave the test tubes in water bath of 30�C for 10minutes. * When 10 minutes is over remove the photographic film as this will top the reaction as no substrate is present anymore. * Shake the solution and record observations of colour change.

  2. Experiment To Investigate The Effect Of Concentration On An Enzyme Based Reaction.

    * Temperature of experiment solution - raising the temperature will increase the rate of the reaction as it gives the molecules more energy causing them to move faster, making them more likely to collide successfully to induce a reaction. Further more, as they have more energy they are more likely

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work