The Effect of Enzyme Concentration on Enzyme Activity

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Amy Bowring

The Effect of Enzyme Concentration on Enzyme Activity

Aim: To investigate the effect of a change in enzyme concentration on activity of that enzyme.

The Pancreas produces enzymes through the pancreatic duct to the small intestine which help digest our food. In people who have cystic fibrosis, the pancreatic duct frequently becomes blocked restricting these enzymes. The aim of this investigation is to investigate the effect of a reduction in enzyme concentration on the rate of reaction. In this case the breakdown of protein by protease enzymes.

The independent variable will be a pepsin enzyme concentration which will decrease from 5% to 1%. This is how the enzyme concentration will be reduced.

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The dependant variable is time i.e how long it takes for the enzyme activity to be complete. This will done by measuring the amount of time it takes for the gelatine mass to be broken down and fall through the funnel completely.

The controlled variables (what must be kept the same) are:

  • A standard mixing procedure, by stirring jelly and enzymes only in a clockwise direction a same speed for 15 seconds for each time the experiment is repeated.
  • The gelatine concentration, using the gelatine jelly that has been made all at the same time from the same ...

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