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The effect of enzyme concentration on rate of reaction.

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Introduction

The effect of enzyme concentration on rate of reaction ZAHRA HUSSIEN AS BIOLOGY TO PHIL CODDALL 27/10/03 * Introduction In this experiment I will attempt to investigate the factors that effect the rate of reaction of the enzyme catalyse. Catalyse is an enzyme found in all living cells. It is used for removing Hydrogen Peroxide from the cells. Catalyse speeds up the decomposition of Hydrogen Peroxide into water oxygen. It is able to speed up the decomposition of Hydrogen peroxide because of the shape of hydrogen peroxide molecule. This type of reaction where a molecule is broken down in to smaller pieces is called anabolic reaction. 2H2O2 + yeast =2H2O + O2 Enzymes are proteins that act as catalysts. They are made in cells. A catalyst is something that speeds up a reaction, but does not get used up in the reaction. The substance that enzymes act on is the substrate (hydrogen peroxide). The substance formed by the reaction is the product (water and oxygen) The site on which the enzyme takes place is called the active site. * Aim To see how the amount of yeast affects how much oxygen is given off in 30 second, when the same concentration of yeast (which contains catalyse) ...read more.

Middle

The volume of hydrogen peroxide remains constant through out the experiment. 4. Measure about 2.5 cm3 yeast using syringe and pore it into the boiling tubes. Increase the volume of yeast by 2.5 cm3 in each new experiment. 5. Fix the boiling tube with yeast in the stand using the clamp. Make sure you fix it 45oc. 6. Put the little tube with hydrogen peroxide into the boiling tube. Don't mix the hydrogen peroxide and the yeast till you are ready to take reading. 7. Close the boiling tube. 8. Fix the gas syringe in a stand using a clamp. Use a gas syringe to measure the oxygen produced. It is specially designed to measure gas and it is made from smooth glass, which will allow all the gas to be collected and easily measured. This makes it very precise and accurate and is easy to use. 9. Connect the gas tube and the boiling tube using deliver tube. The rubber delivery tube is used to transport the gas to the gas collector tube to be measured and to minimise the amount of oxygen lost. 10. Get ready pepper and pen to write the reading. 11. Gets ready the stopwatch. 12. Shake gently the boiling tube to mix the hydrogen per oxide with yeast. ...read more.

Conclusion

* To enable this experiment to be completed as accurate as possible, I will repeat it five times and then use an average of all the results to best plot a graph with a line of best fit. * Keep all the variables apart. * The gas syringe only had measurements for 1 whole number this could have affected the results because no decimal places could be used Ways to Improve experiment. I will use an automatic pipette, as it is very accurate and precise as it measures every 1000th ml. Risk Assessment I will wear a laboratory coat and goggles to protect my eyes, skin and clothes as hydrogen peroxide, and some enzymes can be hazardous and harmful. Hydrogen peroxide can be corrosive if strong, and an irritant to the skin and eyes. It should not be swallowed. It can corrode clothes and irritate the skin if not washed off thoroughly. Eye protection should be worn and should be handled with care. Contact with the skin should be avoided and clothing should be protected. You should also mop/clean up any spills. All enzymes are potential allergies and can irritate membranes in the eyes or nose (if inhaled). They may also cause an asthmatic attack. Eye protection should be worn and care should be taken when handling. You should avoid handling, inhaling or contact with the skin wherever possible. They must not be swallowed and spills must be mopped up immediately. ...read more.

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