The effect of enzyme concentration on rate of reaction.

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           The effect of enzyme concentration on rate of reaction

ZAHRA HUSSIEN

AS BIOLOGY

TO PHIL CODDALL

27/10/03

  • Introduction

In this experiment I will attempt to investigate the factors that effect the rate of reaction of the enzyme catalyse.

Catalyse is an enzyme found in all living cells.  It is used for removing

Hydrogen Peroxide from the cells. Catalyse speeds up the decomposition of Hydrogen Peroxide into water oxygen. It is able to speed up the decomposition of Hydrogen peroxide because of the shape of hydrogen peroxide molecule. This type of reaction where a molecule is broken down in to smaller pieces is called anabolic reaction.

2H2O2  + yeast =2H2O + O2

Enzymes are proteins that act as catalysts. They are made in cells. A catalyst is something that speeds up a reaction, but does not get used up in the reaction.

The substance that enzymes act on is the substrate (hydrogen peroxide).

The substance formed by the reaction is the product (water and oxygen)

The site on which the enzyme takes place is called the active site. 

     

  • Aim

To see how the amount of yeast affects how much oxygen is given off in 30 second, when the same concentration of yeast (which contains catalyse) is mixed with 10cm3 of hydrogen peroxide.

  • Variables

A variable is anything I can change or measure in my experiment.

1) Independent variables

    The one you change through ought the experiment.

   Volume of yeast: - change the amount of yeast in each experiment to find out the effect of it on the rate of reaction.

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2) Dependent variable

The amount of products from each experiment that is water and oxygen.

3) Control variables

These is the variable must stay the same throughout the experiment to ensure a fair test:

Temperature: - It is vital that the temperature remains the same in all the experiments, as this will have a great impact on the results if not.

pH Level:- This is again like temperature in the way that every enzyme has an optimum temperature the same as they have an optimum pH.

Concentration of substrate (H2O2)  :-I will use the same amount and the same concentration of ...

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