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The effect of enzyme concentration on the activity of amylase.

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Introduction

The effect of enzyme concentration on the activity of amylase. I am going to test in the effect of the concentration of the enzyme, the only thing that I should be changing in each experiment is the concentration of the enzyme and nothing else. This will make all of the tests identical (apart from the enzymes concentration) which means the experiment should be accurate and fair. Because of this any differences in the results of my experiment I will be able to put down to the concentration of the enzyme. If I was to use two variables, and there were a difference in the results, e.g. I changed the temperature as well as the concentration of the enzyme and the reaction sped up, I would not know which of the factors had done this and therefore could not reach a valid conclusion. ...read more.

Middle

Surface Area - Reactions can react faster when solids are cut into smaller pieces. This is because there is more surface area which is exposed. The more surface area there is, the more collisions that take place between particles so the reaction rate is much quicker. Enzymes are biological molecules that catalyze many different chemical reactions. With few exceptions, all enzymes are proteins and each enzyme is specific to a certain chemical reaction. Enzymes must maintain a specific three dimensional structure in order to function properly. If an enzyme's structure is altered (by heat or harsh chemicals) it may not function at all. This breakdown (denaturation) of an enzyme's structure may be fatal (your body cannot withstand a fever over 108 degrees for very long). The enzyme I used in this experiment is amylase, which is commonly found in saliva and germinating seeds. ...read more.

Conclusion

Then pipette 5cm� of undiluted enzyme solution and 5cm� of starch solution into another test tube. Stand both tubes in the water bath and leave for several minutes to reach the temperature of the water bath. Take the tubes out and mix the two solutions together, then replace the mixture into the water bath and start the stopwatch. At intervals of one minute, remove a drop of the mixture in the water bath and test it with iodine solution on a white tile. Continue the experiment until the mixture fails to give a blue-black colour with iodine solution. Repeat this procedure with the other concentrations of amylase, using the same amount of each solution. Results Amylase concentration/cm� Time of the achromatic point/minutes 0.01 2.0mins 0.005 3.0mins 0.0033 6.0mins 0.0025 15.0mins ...read more.

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