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The Effect of Enzyme Concentration on the Rate of Reaction

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Elmaz Korimbocus The Effect of Temperature on Protease And Protease and the Effect of pH And The Effect of Enzyme Concentration on the Rate of Reaction AIM There are three different experiments that I will carry out to discover what effect temperature and pH has on the enzyme, protease. The third experiment is to show what effect concentration of the enzyme, urease, has on the rate of reaction. All these things will depend on certain things. INTRODUCTION Enzymes are biological catalysts, which speed up reactions, which goes on inside living things. Without them life would be very slow! The enzymes that I will be using in my experiments are protease and urease. With the protease, I will be testing the effect of temperature on the enzyme and also the effect of pH on it. Protease is used for various things, from the tenderizing of meat to breaking down proteins in baby foods. Pancreatic secretion also contains inactive protease. Urease is found in large quantities in jack beans, soybeans, and other plant seeds. The enzyme, urease, also catalyzes the hydrolysis of urea, forming ammonia and carbon dioxide. It also occurs in some animal tissues and intestinal micro-organisms. APPARATUS AND DIAGRAMS For the first experiment, the effect of temperature on protease, the following equipment was used:- * boiling tubes * test-tube rack * wooden spills * film * protease * measuring cylinder ...read more.


3) This was left for at least 5 minutes, and then removed and then allowed to cool. This sample was referred to as boiled urease. 4) Four test-tubes were labeled from 1-4, and using the measuring cylinder, 5cm3 of urea solution was added. 5) 5 cm3 of acetic (ethanoic acid) solution was also added to the boiling tubes as well as 10 drops of Universal Indicator. 6) To tube 4, 3cm3 of boiled urease was added. 7) To tube 1, 2cm3 of unboiled urease was added. 8) To tube 2, 3cm3 of unboiled urease was added. 9) To tube 3, 5cm3 of unboiled urease was added. 10) I then started the stop clock. 11) At 30 seconds intervals, I shook the tubes and observed the colour of the indicator in each tube. The observations continued for 5 minutes. 12) At each observation, the pH of each of the mixtures was recorded. RESULTS The results of the first experiment of the effect of temperature on protease is as follows, in form of a table:- Temperature of water bath ( C) Time for film spill to go clear 0 23 minutes 05 seconds 20 9 minutes 12 seconds 30 7 minutes 15 seconds 40 6 minutes 38 seconds 50 6 minutes 16 seconds As you can see from the results, the experiment of the effect of temperature on protease is quite conclusive. ...read more.


Enzymes, which act on proteins, are called proteases. I did expect the same type of response and trends to protease and the effect of pH, that I got from the effect of temperature on protease, however the results from the experiment was anomalous. I expected that the higher the pH of the buffer solution, the less time it would take for the enzyme to work and make the film spill clear. How ever, this was not what I obtained. The result that I got from the third experiment was what I expected. The larger the volume of unboiled urease, and longer it was left, the higher the pH would be. There were no anomalous results. The above picture illustrates how enzymes work. Molecules are constantly moving about and bumping into each other. When a substrate molecule bumps into a molecule of the right enzyme, it its into a depression on the surface of the enzyme molecule. This depression is called the active site. The reaction takes place and the molecules of product leave the active site, freeing it for another substrate molecule. The active site of a particular enzyme has a specific shape into which only one kind of substrate will fit. The substrate fits into the active site like a lock and key. This is why enzymes are specific in their actions. ...read more.

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