• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The effect of pH on the activity of catalase

Extracts from this document...

Introduction

The effect of pH on the activity of catalase Introduction Enzymes are biological catalysts. Catalase, an enzyme found in many tissues, catalyses the breakdown of hydrogen peroxide (H2O2) into water and oxygen. It is one of the fattest known enzymes. 2H2O2 catalase O2 + 2H20 Enzymes speed up reaction but not involved in the reaction. They provide a surface for reaction to occur. They also work very rapidly in reactions. Observation Enzymes are globular proteins that increase the rate of specific biochemical reactions; they act as organic and biological catalyst. Enzymes are specific for the reaction they catalyst. Each enzyme operates on a limited number of substrates of similar to generate product under well-defined conditions of concentration, pH, temperature etc. Hypotheses I predict that catalase will work at its optimum at pH 7 to 8. The optimum pH for most enzymes is neutral that is pH 7.Catalase will be denatured at in acidic conditions as the globular three dimension shape will be tortured and permanently be destroyed. Requirements This experiment will be successful done with these materials: Buffer solutions at pH 4.4, pH 5.2, 6.5 and pH 7.5.you will also need a pod, a burette, a pipette, Hydrogen peroxide, syringe, stop clock, test tubes, stoppers, Safety measures Hydrogen peroxide is an irritant product; it bleaches the skin instantly as it gets to it, therefore extra care must be taken when using it. ...read more.

Middle

7.7 5.4 12 9.27 90 9.1 11.8 15.3 7.9 9 6.3 13.2 10.37 105 9.9 12.3 16.1 9.1 10.2 7.2 14.3 11.30 120 10.7 12.8 16.6 10.1 11.2 8 14.8 12.03 135 11.5 13.2 17 11.1 12.1 8.5 15.7 12.73 150 12 13.5 17 11.7 12.7 9.1 16.2 13.17 165 12.4 13.8 17.7 12.4 13.4 9.6 16.6 13.70 180 12.8 14.1 17.9 13 13.9 9.8 17.1 14.09 ph7.5 Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 Mean Time (s) Volume Volume Volume Volume Volume Volume Volume Volume 0 0 0 0 0 0 0 0 0.00 15 3.1 7.2 3.9 1.3 2.9 2.4 5 3.69 30 7.7 11.5 9.9 3.1 8.2 4.4 9 7.69 45 10 13.4 12.9 5.9 11.4 7 11.9 10.36 60 11.7 14.4 14.9 8.1 13.3 9.2 13.5 12.16 75 12.9 15 16.2 9.9 14.7 11 14.6 13.47 90 13.7 15.5 17.1 11.3 15.7 12.3 15.5 14.44 105 14.4 15.9 17.5 12.4 16.4 13.4 16.2 15.17 120 15 16.1 18 13.3 16.9 14 16.8 15.73 135 15.5 16.5 18.6 14 17.4 14.6 17.2 16.26 150 15.8 16.7 18.8 14.7 17.7 15.1 17.7 16.64 165 16.2 16.8 19 15.2 18 15.8 18 17.00 180 16.5 16.9 19.2 15.7 18.3 16 18.3 17.27 Mean of all the results PH4.4 ph5.2 ph6.5 ph7.5 0 0.00 0.00 0.00 0.00 15 1.29 0.84 1.50 3.69 30 4.23 2.89 3.87 7.69 45 6.27 5.00 6.13 10.36 60 ...read more.

Conclusion

It may also be that the enzyme catalase haven't yet reached its optimum or had passed it optimum slightly, this would have be confirm or dealt with if say pH 7 and pH 8 was used in this experiment. With reference to my error bars on mine graph, it is shown clearly how accurate the class mean was on each pH. It is also shown clearly that if I compare pH's 4.4 and 6.5 to pH's 5.2 and 7.5, I can tell that because the error bars on pH 4.4 and 6.5 are spreadout , the results are less accurate then that of pH's 5.2 and 7.5 which are much much closer. Evaluation Overall I think my hypothesis was correct and the experiment proved it. There are so many factors that could contribute to some improvement in the method. One isa gas syringe could have been used to measure the volume from the burette was turned upside down and many people found it difficult reading the the volume taken at each time interval. Time could also cause a be error as time may be different at diferrent times and redings may be inaccurate. Another cause of an error in this pratical could be reading the volume on the burette, this was because the readings were white and was almost the same colour as the water and therefore made it difficult to read and record accurate volumes. This could be eliminated next time by using burettes with coloured readings when repeating this practical. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    M concentration Experiment 1 Experiment 2 Experiment 3 Average Standard Deviation Coefficient of variation Time (s) Volume (ml) Volume (ml) Volume (ml) Volume (ml Volume (ml) % 30 11.5 11.5 11.0 11.3 0.3 2.5 60 12.5 11.5 12.0 12.0 0.5 4.2 90 14.0 12.5 14.5 13.7 1.0 7.6 120 16.5

  2. Free essay

    Investigating the effect of PH on the activity of the enzyme catalase.

    These 3 variables will be kept the same through out the whole experiment to ensure it becomes a fair test. Temperature - The rate of reaction starts off slow due to insufficient kinetic energy from low temperature. Few collisions between enzyme and substrate.

  1. Investigating the effect of pH on the activity of an enzyme.

    of this will be used as protease, the two volumes will add up to 6cm� this is more than enough for the cuvette, so it an appropriate measure to use. ==> Test tubes: 9 test tubes will be needed as there are 9 different pH to be measured.

  2. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    Summary of Findings: 2H2O2 --> 2H2O + O2 ? An enzyme decreases the activation energy so that the substrate molecules have energy greater than, or can easily acquire enough energy to reach, the new activation energy, and thus the rate of reaction increases.

  1. Investigating the Effect of pH on Enzymes

    The activation energy is that needed to start a chemical reaction. This means that no other extra heat is needed to make the chemical reaction start, i.e. heat, so the cells in the body can work at lower temperatures. The energy diagram below will help explain activation energy.

  2. The effect of Electromagnetic Fields on Enzyme Activity

    important hormone which is also needed in the production of seratonin, an essential neurotransmitter to the nervous system. Another enzyme on which they did some research was ODC (ornithine decarboxylase). This enzyme is always present during cell growth, but increased levels of ODC are considered a marker for the kind of increased cell activity common in cancers.

  1. INVESTIGATING THE EFFET OF pH ON THE ACTIVITY OF IMMOBILISEDAMYLASE

    It reduces the overall cost of the whole process. It can be used in continuous processes, which can be automated. Some immobilized enzymes are more stable than when they are in a free solution; they have less chance of been denatured. Enzymes can be immobilsed in a range of materials, E.g.

  2. An experiment to see the effect of pH on the activity of Catalase

    Hydrogen peroxide = 2H2O2 Hydrogen peroxide + catalase -> oxygen + water The books and resources I used were: Energy and chemistry - p229 Gareth Williams, biology for you pages 29-31 www.google.com - enzymes, catalase, hydrogen peroxide OCR staged assessment phase 1 module by Mary Jones Pre-test: * In the

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work