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The Effect of Substrate Concentration on the Rate of Reaction

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Introduction

The Effect of Substrate Concentration on the Rate of Reaction Aim: The aim is to look at how the rate of reaction in yeast catalase is affected by a particular substrate concentration. The substrate that is used in this experiment is hydrogen peroxide on the yeast catalase, which will bring faster and more precise results. Enzymes only work with substrates that fit their active site, called the lock and key mechanism. This is where the substrate fits into the enzyme in the same way that a key fits into a lock. During the process, a substrate is fitted into an active site of an enzyme. ...read more.

Middle

H2O2 H2O + O2 Apparatus Used: 1. Stop watch. 2. Measuring Scale. 3. Stand with clamps. 4. Gas syringe. 5. Measuring cylinder. 6. Clonical flask. 7. 1.5 grams of yeast catalase. 8. 4 cm� of hydrogen peroxide of various concentrations. Method: 1. 1.5 grams of yeast catalase has to be measured. 2. The 4% hydrogen peroxide concentration is measured to the amount of 4cm�. 3. Put the east catalase inside the clonicle flask and after that, put in the hydrogen peroxide. 4. Start the stopper immediately while watching the gas syringe move along the marks on the side reaching 30cm�. ...read more.

Conclusion

RATE OF REACTION (VOL OF O2/ AVERAGE TIME TAKEN) 0% 00.00 00.00 00.00 00.00 4% 17.16 15.67 16.42 1.82 8% 11.84 9.78 10.81 2.77 12% 8.30 8.90 8.60 3.49 16% 4.69 5.66 5.18 5.79 20% 2.55 3.45 3.00 10.00 24% 2.04 3.00 2.52 11.90 Conclusion: In conclusion, I have witnessed that the higher the concentration of hydrogen peroxide, then the quicker the gas is produced. The reason there is a high rate of reaction is because the formation and collision of the substrate with the enzyme. If the substrate concentration was increased, then the oxygen gas would be produced at a much faster rate. In comparison, lower concentrations will lower collision chances which will, in result, produce less oxygen with an extremely slow rate. ...read more.

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