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the Effect of Temperature on an Immobilised Digestive Enzyme

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Introduction

the Effect of Temperature on an Immobilised Digestive Enzyme Results Temperature % Glucose 1 % Glucose 2 % Glucose Mean Time Ice Bath 0.01% 0.25% 0.13% every 30 0.01% 0.25% 0.13% seconds 0.01% 0.25% 0.13% 0.01% 0.25% 0.13% 0.01% 0.25% 0.13% Room Temp 0.01% 0.00% 0.01% every 30 0.25% 1.00% 0.63% seconds 0.50% 0.25% 0.38% 0.50% 0.25% 0.38% 1.00% 0.50% 0.75% 1.00% 0.50% 0.75% 2.00% 1.00% 1.50% 2.00% 1.00% 1.50% 30oC 0.25% 0.01% 0.13% every 30 0.50% 0.01% 0.26% seconds 1.00% 0.01% 0.51% 2.00% 0.25% 1.13% 2.00% 0.25% 1.13% 2.00% 0.50% 1.25% 2.00% 0.50% 1.25% 2.00% 1.00% 1.50% 45oC 0.01% 0.25% 0.13% every 30 0.25% 0.50% 0.38% seconds 0.50% 0.50% 0.50% 0.50% 1.00% 0.75% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 60oC 0.25% 0.01% 0.13% every 30 0.25% ...read more.

Middle

This would have allowed for anomalous results and would have increased the experiments accuracy considerably. The ice bath run had a very low activity rate. It climbed up to a certain level and stayed constant all the way through the experiment. The room temperature run climbed steadily every minute giving the same result for each 30 second interval throughout each minute. This was true for both glucose percentage run. At 30oC either climbed quicly ans settled at 2% or climbed steadily, resting at 1%.The 45oC run jumped to 1% rapidly and atayed at that terminal point. The highest temperature, 60oC, steadily worked it's way up to a mean average of 1.5%. ...read more.

Conclusion

In an improved version of the experiment, a new set of beads could have been used for each run. Ideally the experiment could have been done with a varying amount of beads and if possible vary the size of the beads by changing the height at which the solution was dropped and by adujusting the choke of the syringe. This would have to be done by phsically widening the syringe mouth of by using a larger syringe. Conclusion From the results the conlusion that the optimum temperature is the room teperature which was approximately 20oC. This conclusion is based on the mean averages and the rate at which the glucose reached it's optimum level. However 30oC was definitely a close second because it too climbed to the optimum quickly, But the mean average was just below that of the room temperature. ...read more.

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