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The effect of temperature on beetroot membranes

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Introduction

%T at 430nm temperature ( °C) Ellen Sophie Luke LucyB Will Claire Average 0 93 93 10 82 113 88 90 91 92.8 20 87 90 92 91 91 89 90 30 72 85 75 93 95 89 84.8 40 62 86 80 82 84 90 80.7 50 93 87 41 52 68 68.2 60 11 14 10 12 16 11 12.3 70 11 13 25 12 10 11 13.7 80 14 11 19.5 90 Beetroot membranes Results Factor Way of controlling/ preventing this factor influencing the outcome Scientific reason to explain why this factor could make a difference to my results How my results will be affected Type of error introduced Size/surface area of beetroot sections I could use a size 4 cork borer to make sure all pieces of beetroot are the same size, and a ruler and knife to cut 1 cm length slices, so that they are all the same length. ...read more.

Middle

This is the control reading for the colorimeter. It would affect the dilution of the dye, so exactly the same volume of water would have to be used in each test tube in order to eliminate discrepancies in colour of pigment-water solution due to differing amounts of dilution. I would see an inconcordant reading for absorbency of light, if the dye is too diluted compared with the control readings. Random because it is an error in measurement of volume of water that would lead to inconsistent results Independent variable The variable to be adjusted is the temperature and will be adjusted by using water baths of different temperatures to put the test tubes in. Dependent variable The results will be measured by a colorimeter which will measure the absorbency of the liquid to show how much beetroot has leaked out. We repeated the experiments until we found concordant results as it means results will be more reliable, and will make sure that none of our results are anomalous. ...read more.

Conclusion

However, we did get some anomalies due to random and systematic errors. This is because as the temperature increases so does the amount of kinetic energy which will denature the proteins embedded in the phospholipid bilayer. This allows the pigment in the beetroot to diffuse out of the membrane through osmosis where the proteins have been denatured. Two of our results are anomalous, our reading of 93 %T at 430nm at 0 degrees C, and our reading of 25 %T at 430nm at 70 degrees C. These anomalies are probably due to a random error, such as taking down the reading incorrectly by mistake. Also, from the classes average results our results for 60, 70 and 80 degrees C were the wrong way round as to what I?d predicted in my hypothesis because we thought the higher the temperature the higher the absorbency would be. This is probably due to the fact that its a class average and there would be slight variations between groups on how the experiment was carried out. ...read more.

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