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The Effect of Temperature on Enzymes

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Introduction

The Effect of Temperature on Enzymes I am going to investigate the effect of temperature on enzymes by carrying out a series of experiments. I will work with one variable - the temperature, but apart from that each experiment will be exactly the same in order to ensure that it is a fair test. Apparatus: test tubes, test tube rack, 10ml syringe, dimple tiles, pipette, iodine solution, 250ml beaker, water bath, starch solution, stop clock, glass rod, thermometer. In order to carry out the experiment do the following: Use the clean syringe and put 10ml of starch solution into a test tube, (make sure there are no air bubbles as this may alter measurements and bias results). Use a different syringe to put 1ml of amylase into another test tube and label it, (this prevents contamination of the substances, which could bias overall results. Stand both test tubes in the water bath for five minutes and record the temperature. Care must be taken when using the water bath as electricity can be dangerous when in contact with water. Whilst waiting, put two drops of iodine solution into each dimple on a dimple tile with a pipette however, take care not to allow the solution to come into contact with eyes. ...read more.

Middle

This also helps me in that I know that if any of the experiments I am planning to do at higher temperatures do not work, it will be because the substrate (starch) will not fit inside the enzyme (amylase) to be broken down. I can also predict that the experiment will not work at extremely low temperatures because the enzymes will not have enough energy to meet their substrate molecules. I think that the experiment will work best at around room temperature as I have referred to the following worksheet, BIOTECHNOLOGY 2 HOW ENZYMES WORK. From all the research I have done, I can predict that overall; my results will follow a trend in that the enzymes will cease to work at very low temperatures, the rate at which they work will gradually increase up until around room temperature, and then decrease until they get up to high temperatures, where they will also cease to work. In order for my prediction to have any sort of meaning however, the experiments must be carried out accurately and fairly, otherwise I may obtain biased results which do not fit the normal pattern. After doing this experiment it is clear that the reaction proceeds at different rates at different temperatures, however after drawing two graphs it is also clear that it follows a significant trend. ...read more.

Conclusion

There are many other reasons why one of my results could have been anomalous, however I know scientifically that the result should have been closer to the trend line and that it must have been through a mistake of my own that it wasn't. Although my results give a rough idea of the points where all the enzymes become denatured, where the enzymes do not have enough energy to work and which is the best temperature for this reaction to take place, it is impossible to say exactly where these points are. This is because I have experimented with temperature intervals of mainly ten degrees, and if I wanted to find out the exact temperatures I would need to experiment with perhaps one degree above and below the temperature that I did use. It is also possible that the enzymes do react at zero degrees, just extremely slowly. This is why I would like to repeat the experiment, however observing for forty minutes as opposed to just twenty. If I do repeat the experiment I will probably do it more times in order to find a more accurate average and therefore be less likely to come across an anomalous result. Even though my results aren't totally reliable I feel that they can support a firm conclusion because they follow the same pattern that I learned about earlier when doing my research. Danielle Jones 10m ...read more.

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