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The effect of Temperature on membrans

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Introduction

The effect of temperature on the membranes For this experiment, I will be testing the effect of temperature on the membranes of beetroot. Beetroot cells are like any other eukaryotic cells, and have just like any other cell, the standard organelles present needed for the cell to function correctly. Some of the organelles are bounded by a single membrane, while others are bounded by two membranes. The aim of this experiment is to test how different temperature will affect the overall structure of the membrane, and how the beetroot will be affected by the varying temperature. I will test how by heating beetroot slices of the same size at a varying temperature how the heat affects the cell membranes. The independent variable will be the temperature of the water surrounding the beetroot slices, because I have full control over this variable, while the variable that I will keep constant will be the size of the 8 pieces of beetroot that I'll use. The dependant variable that will occur will be the effect on the beetroot as it is dependant on the temperature if a higher or lower temperature has an effect on the slices. ...read more.

Middle

30 minutes is enough for the temperature to fully boil and have a full affect on the membrane of the beetroot. 6) After the 30 minutes, remove the boiling tubes and shake the test tubes to liquefy the pigment in the water 7) Place them in the test tubes racks 8) In order to document the change, one uses the pipette to measure 2cm^3 of the water and dye solution. 9) Place each sample into the colorimeter and measure the absorbency of each beetroot as shown on the monitor on the colorimeter. Below are the results I obtained from the experiment. Temperature How much it absorbed 0 0.17 10 0.1 20 0.06 30 0.07 40 0.1 50 0.25 60 0.3 70 1.15 From this set of results, I manipulated them into a visual graph in order to see the results a lot more clearly. 0 10 20 30 40 50 60 70 By looking at my results, one can see that there is a steadily increasing rate of percentage absorbed correlating with the temperature increased. The amount absorbed was measured by the colorimeter, in which is a reliable measurement to base my recordings on. ...read more.

Conclusion

To avoid this next time, there should be more than one person working on the experiment in order to collect an accurate account of results. There were also many random errors that may have occurred during the conduction of my experiment. Because the readings on the colorimeter were very reliable and accurate as it was a machine reading, I felt fully confident that these results were as accurate as they could be. The only other thing that I could have easily got wrong was when I was meant to shake the test tube gently. I had no general idea how much I was shaking each test tube to mix up the dye and pigment solution, and could have shaken one test tube more than the other, giving some of them an unfair reading on how much it had been absorbed. Furthermore, the colorimeter, although I'm very sure it was fine, could have not been functioning properly. Taking this into consideration, I could have repeated the experiment to obtain much more results. Overall, I can conclude by saying that this experiment proved my prediction with the use of biological knowledge and reliable results. I can successfully conclude by saying that the change in temperature will change the overall structure in the beetroot membrane by making it more permeable the more the temperature increases. ...read more.

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