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The effect of Temperature on the activity of an enzyme.

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Introduction

The effect of Temperature on the activity of an enzyme Background information An Enzyme is a special type of protein that is found in the membrane systems in the body. Enzymes help us digest food chemicals like carbohydrates and lipids. Enzymes are a form of catalyst, a catalyst is a chemical that speeds up a reaction but is not used up by it. In this experiment, the enzyme used will be trypsin and the substrate will be milk. Trypsin is an enzyme that digests protein, in this case the proteins in milk. Trypsin is one of the proteases from the pancreas; it is secreted as the inactive trypsinogen and is activated by enterokinose, an enzyme secreted by the line of the duodenum. ...read more.

Middle

I predict that he optimum temperature is around 37.5 degrees Celsius because that is the temperature in our body. Apparatus 1) Boiling Tube - to carry out the experiment. 2) Trypsin - Enzyme. 3) Milk - Substrate. 4) Water - to mix the milk powder. 5) Stopwatch - To time the experiment. 6) Measuring Cylinder - To measure the amount of Liquid needed. 7) Thermometer - To measure the Temperature of the solution. 8) Water bath(s) - To heat up the solution. Variables 1) Temperature (Will be changed) 2) Volume of Enzyme (Will not be changed) 3) Volume of Substrate (Will not be changed) 4) Concentration of Enzyme (Will not be changed) 5) Concentration of Substrate (Will not be changed) ...read more.

Conclusion

The same solution of enzyme and substrate will be used throughout the whole experiment; this ensures the concentration of the substances to be kept the same. The pH should be kept the same but there is nothing to keep it at a constant like a buffer solution. Other Factors Apart from the Variables above, there is other factor that might affect the outcome of this experiment. Human faults will affect the results, we may be undecided to whether the milk is clear or not. Also, the amount of Enzyme/Substrate poured out could be measured inaccurately; this will affect the results. Also, residue inside the boiling tubes may affect the speed of the reaction so the results might be inaccurate. To minimise these factors from affecting the experiment, the experiment will be carried out care fully and will be repeated twice each and the average will be found out. ...read more.

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