• Join over 1.2 million students every month
• Accelerate your learning by 29%
• Unlimited access from just £6.99 per month
Page
1. 1
1
2. 2
2

# The effect of temperature on the activity of trypsin.

Extracts from this document...

Introduction

The effect of temperature on the activity of trypsin Method: To begin the experiment safety precautions were carried out, goggles and a laboratory coat was put on. A water bath had been previously prepared for us at 30c. Three test tubes were labelled '30c casein', '30c trypsin' and '30c control'. Using a pipette, 5cm? of casein suspension was pipetted into each of the test tubes labelled '30c casein' and '30c control'. Using another pipette to avoid contamination, 5cm? of trypsin solution was pipetted in the test tube labelled '30c trypsin'. And again, using a different clean pipette, 5cm? of distilled water was added into the test tube labelled '30c control'. The three test tubes were then placed into the water bath and left for several minutes to reach the temperature of the water (we tested this by placing thermometers in each test tube, we had to use three thermometers because if one thermometer was transferred from one tube to the other it would have contaminated and thus started the reaction). The enzyme (the trypsin) was then added to the substrate (the casein) and agitated to ensure that they were thoroughly mixed. This was then replaced in the water bath and a stopwatch was started immediately. The contents of the tube were then carefully observed (and every now and again the tube was agitated to keep the contents moving) ...read more.

Middle

0.0048 = 0.48 x 10 -2 Analysis: The two lines of my graph are similar and yet also different. At first both of the lines rise, this indicates that as the temperature is increased the time taken for the suspension to clear became less (the rate of reaction increases). But after a certain point they both differ; my personal line goes up, suggesting that the time taken for the suspension to clear became even quicker, but the class line goes slightly down, which suggests that the time taken for the suspension to clear was slower. My line shows a pattern in which as the temperature increases the enzyme activity increases. The class line shows that the activity of the enzyme will increase if the temperature increases, but only within a certain temperature, after that it will then start to decrease. Using my knowledge of the effect of temperature on enzymes I can understand why there are differences in my two lines. If other factors such as pH, enzyme concentration and substrate concentration are kept constant then temperature has an effect on enzyme activity. At the beginning, a rise in temperature will increase the kinetic energy of the enzyme and the substrate molecules, and will therefore tend to increase the rate of a chemical reaction. ...read more.

Conclusion

This is due to the fact that the lid was taken off of it and also more and more cold test tubes from each group were going into it so the energy went into heating the tubes up. A difference in temperature could affect the results of the experiment. * Some groups were treating the test tubes differently from others: after when the enzyme has been added to the substrate some groups remembered to regularly agitate them to ensure that they were still moving, other didn't mix the tubes thoroughly and were left with a substance that was clear at the top but still cloudy at the bottom. Some groups also accidentally contaminated the test tubes by using one thermometer for the trypsin and the casein. Considering all of these factors, it explains why my personal line was different from the class line. If this experiment were to be carried out again I would try and make improvements: 1. A shared water bath is not ideal as the temperature of the water bath will drop and affect the results. Individual water baths should have been used. The temperature of the test tubes needs to be accurate in order to produce accurate results and a graph. 2. The test tubes need to be agitated regularly, as the milk is denser than trypsin and could sink to the bottom of the test tube and also contamination needs to be avoided at all costs. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

## Found what you're looking for?

• Start learning 29% faster today
• 150,000+ documents available
• Just £6.99 a month

Not the one? Search for your essay title...
• Join over 1.2 million students every month
• Accelerate your learning by 29%
• Unlimited access from just £6.99 per month

# Related AS and A Level Molecules & Cells essays

1. ## Investigate the effect of temperature on the activity of trypsin, using a suspension of ...

The combination of enzyme and substrate crease a new energy profile, for the reaction. Activation Energy Without Enzyme Energy Level Activation Energy With Enzyme. Progress of Reaction This graph illustrates how addition of an enzyme lowers the amount of activation energy required to initiate the reaction.

2. ## The aim of the experiment is to investigate the effect of temperature on the ...

Enzymes are usually very large in comparison with substrate, so only small portion of amino acid residues are near or in direct contact with substrate in enzyme-substrate complex. Most of the enzyme involved in control and maintaining correct structural configuration of enzyme i.e.

1. ## Investigate the effect of enzyme temperature on the activity of the enzyme Trypsin on ...

This transition state has a higher energy level than either the substrates or the product. Outside the body, a lot of heat is used to initiate the reaction. However, this is dangerous inside the body, so we have enzymes that an alternative way with a different transition state and lower activation energy.

2. ## The effect of temperature on the activity of trypsin.

Results Temperature C Time (seconds) 30 479 40 337 50 125 60 102 Class results Temperature C Time (seconds) Average (seconds) 30 714 476 450 479 352 452 830 536 40 330 215 384 337 165 366 622 346 50 114 190 171 125 155 267 130 165 60 154

1. ## Investigation in to the effect of temperature on the activity on the enzyme Trypsin.

Any other possible variables such as enzyme concentration and pH must be kept invariable to ensure more accurate results from the experiment. HYPOTHESIS: The kinetic theorem states that all particles are in constant motion and always possess a certain amount of Kinetic Energy.

2. ## The effect of Electromagnetic Fields on Enzyme Activity

There is a fairly new theory on how animals and humans are able to sense the earth's electromagnetic field. It is thought that they are able to perceive it by the use of magnetite which has been discovered in most animals.

• Over 160,000 pieces
of student written work
• Annotated by
experienced teachers
• Ideas and feedback to