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The Effect Of Temperature On Trypsin Enzymes

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Biology Investigation: The Effect Of Temperature On Trypsin Enzymes Plan Introduction I am going to be conducting an experiment to find out how temperature effects the rate at which trypsin hydrolyses protein found in milk.. Trypsin is secreted by the pancreas of most warm blooded animals into the small intestine. It is a biological catalyst, speeding up or slowing down the rate at which protein is broken down. I will test how quickly the trypsin works at a series of different temperatures ranging from 10 to 90�cetigrade Hypothesis I predict that the optimum temperature, that I am testing, that the trypsin will work at is 40�c. This is because 40�c is nearest to the body temperature of warm blooded animals. So the trypsin will be accustomed to working at this temperature. I also predict that when I test the trypsin at 90�c it will not work at all. This is because trypsin is a living enzyme, once it has been heated to 90�c it probably will have denatured. It's shape will have become distorted and the substrate (the protein in this case) will not fit into the active site. I found the information that I have used for this prediction in the Co-ordinated Science 1 text book written by Christopher Lale, Ann Daniels and Mark Duke. Lock and key theory The lock and key theory is a simple theory explaining the way that an enzyme breaks up its substrate. ...read more.


3. Separately measure out 5ml of trypsin and 5ml of milk into test tubes. Put these tubes up-right in the beaker of water, then put the thermometer into the water and add ice cubes until the temperature on the thermometer drops to 10�c. 4. At this point mix the milk and trypsin and start the stopwatch at the same time. Put the test tube containing the mixture next to the control test on the test tube rack and compare it. As soon as it is as clear as the control, stop the stopwatch and write down the time on the results table. 5. Separately measure out 5ml of trypsin and 5ml of milk into test tubes again. Take the ice cubes out of the water and put the test tubes upright in the water wait for the temperature on the thermometer to reach 20�c and then repeat step 4. 6. Once again, separately measure out 5ml of milk and 5ml of trypsin into test tubes and put the tubes upright in the water. Set up the bunson burner, tripod, gauze and safety mat. Put the beaker over a heating flame on top of the gauze. Heat to 30�c and then repeat step 4. 7. Repeat step 6 replacing 30�c with 40�c, 50�c, 60�c and finally 90�c. 8. Repeat steps 3-7 inclusive two more times. Results Table: Temperature Test 1 Test 2 Test 3 Average 10�c 8:09 8:30 8:37 8:25 20�c 4:09 4:24 4:27 4:20 30�c 3:00 2:06 2:02 2:23 40�c 1:05 ...read more.


This outstanding result has pulled the average up slightly too, but it does not matter very much because it is not so outstanding that I would draw any different conclusions because of it and I also have two other sets of results to go by anyway. Possible improvements 1. If I'd had more time, Instead of just testing 10�c,20�c,30�c,40�c,50�c,60�c and 90�c I could have tested 70�c and 80�c as well. This way I would have been able to tell more accurately at which temperature the trypsin enzyme denatured. 2. If I'd had lots more time I could have tested at more regular temperature intervals. This would have given me more reliable results. 3. With better equipment I could have put a light one side of the test tube and a light sensor the other side. I could have set the sensor to time how long it took before it was sensing a certain level of light (the level of light could be equal to the amount allowed through by the control test). This way I would not have had to rely on my own human judgement so I would have got more accurate results. However, I still think that the experiment went well and my results were reliable and accurate enough to draw a clear conclusion. I gathered the information that I worked with during this experiment from: - My Co-ordinated Science 1 textbook, written by Christopher Lale, Ann Daniels and Mark Duke (section 7 supplying the cell and biology section) - Teaching given to me over the course of the past year. 1 Anthony Tribe Biology Coursework ...read more.

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3 star(s)

This is a good practical write up which include some good, reliable results. The graph was not included so the analysis is hard to comment on. 3 stars.

Marked by teacher Louise Star 09/04/2013

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