• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The effect the concentration of Hydrogen Peroxide has on the rate of reaction when catalase is added to it.

Extracts from this document...


The effect the concentration of Hydrogen Peroxide has on the rate of reaction when catalase is added to it. Planning Research Hydrogen Peroxide is a chemical compound of hydrogen and oxygen with the formula H2O2. It has many uses such as the bleaching of hair, ivory, feathers, and delicate fabrics, which would be destroyed by other agents. It is also used medicinally as an antiseptic and throat wash. Hydrogen peroxide is used in restoring the original colours to paintings that have darkened through the conversion of the white lead used in the paintings to lead sulphide. It is a poison produced by every living cell. Cells use an enzyme called catalase to break it down into water and oxygen. Hydrogen Peroxide Water+Oxygen 2 H2O2 2 H2O + O2 An enzyme is a biological catalyst. They speed up the rate of a reaction however they are not affected themselves whilst doing this, this is why they are catalysts. Enzymes are made to be specific, this means that they can have only one substrate that they will work on. Each enzyme has an active site that is where their own specific substrate's molecule will fit into. ...read more.


This Proved to be fine and it was easy to measure the results. Now I need to try the concentration at 10 ml Hydrogen Peroxide and no water still keeping the other variables the same. This also worked well so I decided the final values to test will be: Amount of Hydrogen Peroxide (ml) Amount of Water (ml) Amount of Catalase (ml) Temperature (�c) Time (seconds) 5 ml 5 ml 10 ml 21�c 120 secs 6 ml 4 ml 10 ml 21�c 120 secs 7 ml 3 ml 10 ml 21�c 120 secs 8 ml 2 ml 10 ml 21�c 120 secs 9 ml 1 ml 10 ml 21�c 120 secs 10 ml 0 ml 10 ml 21�c 120 secs Apparatus o Bowl of water o Conical Flask o Bung and delivery tube o Measuring Cylinder o Stop Watch/Clock o Clamp and Clamp Stand o Hydrogen Peroxide o Water o Catalase Method o Set up apparatus as shown o Measure out and mix the Hydrogen Peroxide and water in the amounts needed in table above e.g. 5ml Hydrogen Peroxide and 5ml of water and put it in the conical flask. ...read more.


The first reason why the experiment wasn't entirely accurate was when the catalase was added to the H2O2 there will always be a space of time between the catalase entering the H2O2 and the bung being replaced. Therefore some of the O2 will have already escaped. Another problem with the experiment was keeping the temperature constant throughout the experiment. If the temperature fluctuated slightly the reaction could have speed up/slowed down therefore producing anomalous results. Another problem was how the gas was measured. Because it was measured in an upside down measuring cylinder results can easily be misread. Also the large scale on the measuring cylinder also made it difficult to read results accurately. This meant tat many of the results were rounded up or down to nearest readable measurement so the results weren't precise. I could have improved the experiment to get more accurate results if I change the following things. I could have used a smaller measuring cylinder with a smaller scale so the results were more accurate and not rounded up to the nearest readable measurement. I could have also changed the concentration in smaller amounts at a time for e.g. increasing the concentration by 0.5ml at a time rather than 1ml. Finally I could have conducted 4 or 5 times in order to calculate a more accurate average. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    Apparatus often varies slightly in volume and performance rate. For example, the same measuring cylinder should be used because the ease at which gas can enter the cylinder varies (performance rate), and also the graduations may vary slightly. This variable must be kept constant in order to abide by the fair test criteria.

  2. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    Variables Below are comments on the variables which must be controlled to ensure a fair experiment is being conducted. Pressure: The pressure is effectively controlled in the classroom where we are doing the experiment in. If it was not, then we would do the experiment in a pressure-controlled chamber.

  1. Reaction of Catalase and Hydrogen Peroxide

    When the enzymes begin to approach the maximum rate at which they can combine with reactants and release products, the effects of increasing substrate concentration diminish. At the point at which the enzymes are cycling as rapidly as possible, further increases in substrate concentration have no effect on there reaction rate.

  2. Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with ...

    Another preliminary experiment that I did was to check how the strength of the catalase solution used, affects the rate at which hydrogen peroxide breaks down into water and oxygen. I did this experiment to find out the best strength of the catalase solution so that I could use this strength in my temperature investigation.

  1. Investigating the break down of Hydrogen Peroxide using celery tissue to supply the enzyme ...

    Catalase is an enzyme found in food such as potato, celery and liver. It is used for removing Hydrogen Peroxide from the cells. Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen. It is able to speed up the decomposition of Hydrogen Peroxide because of the shape of the Hydrogen Peroxide molecule.

  2. An Investigation on the Effect of Enzyme Concentration on rate of hydrogen peroxide breakdown.

    The method by which I plan to achieve this is that the hydrogen peroxide will be prepared to a concentration which does not need to be specific but will have a high concentration as to provide enough substrate molecules. All the experiments will use hydrogen peroxide from this batch.

  1. Investigating the effect of substrate concentration on reaction between Hydrogen Peroxide (H2O2) and the ...

    An enzyme catalyses a reaction by lowering the activation energy, so less energy is needed for the reaction to take place. The graph below shows the difference between activation energies in a reaction when there is an enzyme present and when there is no enzyme present: As the diagram shows,

  2. the effect of catalase concentration on the breakdown rate of h2o2

    After this change has occurred the product/s renounce the active site, leaving the enzyme molecule unchanged, ready to be made use of again. The following equation is representational of the progress of an enzyme-catalysed reaction; E + S - ES - EP - E + P E = enzyme S

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work