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The effect the concentration of Hydrogen Peroxide has on the rate of reaction when catalase is added to it.

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Introduction

The effect the concentration of Hydrogen Peroxide has on the rate of reaction when catalase is added to it. Planning Research Hydrogen Peroxide is a chemical compound of hydrogen and oxygen with the formula H2O2. It has many uses such as the bleaching of hair, ivory, feathers, and delicate fabrics, which would be destroyed by other agents. It is also used medicinally as an antiseptic and throat wash. Hydrogen peroxide is used in restoring the original colours to paintings that have darkened through the conversion of the white lead used in the paintings to lead sulphide. It is a poison produced by every living cell. Cells use an enzyme called catalase to break it down into water and oxygen. Hydrogen Peroxide Water+Oxygen 2 H2O2 2 H2O + O2 An enzyme is a biological catalyst. They speed up the rate of a reaction however they are not affected themselves whilst doing this, this is why they are catalysts. Enzymes are made to be specific, this means that they can have only one substrate that they will work on. Each enzyme has an active site that is where their own specific substrate's molecule will fit into. ...read more.

Middle

This Proved to be fine and it was easy to measure the results. Now I need to try the concentration at 10 ml Hydrogen Peroxide and no water still keeping the other variables the same. This also worked well so I decided the final values to test will be: Amount of Hydrogen Peroxide (ml) Amount of Water (ml) Amount of Catalase (ml) Temperature (�c) Time (seconds) 5 ml 5 ml 10 ml 21�c 120 secs 6 ml 4 ml 10 ml 21�c 120 secs 7 ml 3 ml 10 ml 21�c 120 secs 8 ml 2 ml 10 ml 21�c 120 secs 9 ml 1 ml 10 ml 21�c 120 secs 10 ml 0 ml 10 ml 21�c 120 secs Apparatus o Bowl of water o Conical Flask o Bung and delivery tube o Measuring Cylinder o Stop Watch/Clock o Clamp and Clamp Stand o Hydrogen Peroxide o Water o Catalase Method o Set up apparatus as shown o Measure out and mix the Hydrogen Peroxide and water in the amounts needed in table above e.g. 5ml Hydrogen Peroxide and 5ml of water and put it in the conical flask. ...read more.

Conclusion

The first reason why the experiment wasn't entirely accurate was when the catalase was added to the H2O2 there will always be a space of time between the catalase entering the H2O2 and the bung being replaced. Therefore some of the O2 will have already escaped. Another problem with the experiment was keeping the temperature constant throughout the experiment. If the temperature fluctuated slightly the reaction could have speed up/slowed down therefore producing anomalous results. Another problem was how the gas was measured. Because it was measured in an upside down measuring cylinder results can easily be misread. Also the large scale on the measuring cylinder also made it difficult to read results accurately. This meant tat many of the results were rounded up or down to nearest readable measurement so the results weren't precise. I could have improved the experiment to get more accurate results if I change the following things. I could have used a smaller measuring cylinder with a smaller scale so the results were more accurate and not rounded up to the nearest readable measurement. I could have also changed the concentration in smaller amounts at a time for e.g. increasing the concentration by 0.5ml at a time rather than 1ml. Finally I could have conducted 4 or 5 times in order to calculate a more accurate average. ...read more.

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