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The experiment we are testing is to see how the surroundings of the amylase and starch affect the rate of reaction and how starch changes amylase into simple sugars.

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Introduction

Introduction: The experiment we are testing is to see how the surroundings of the amylase and starch affect the rate of reaction and how starch changes amylase into simple sugars. Enzymes act as biological catalysts and are specific in the reactions they catalyse. Usually an enzyme will just catalyse only one type of reaction. It only fits into one specific pattern and each enzyme has its own substrate. Biological catalysts are produced in cells and capable of speeding up the chemical reactions. These are simple proteins of a large size and are specific in each chemical reaction needing its own individual enzyme. Enzymes are speeded up by different factors like temperature and pH. Catalyst's increase the rate of a chemical reaction but is never used up during the reaction. In this experiment we will look for starch and see if sugars are present at different temperatures. ...read more.

Middle

5.Control the variables 6.The same volumes of concentration Safety: To make sure the test is safe we must follow these procedures. 1.Use test tubes safely and correctly. 2.Any spills should be cleaned up immediately to avoid accidents 3.Wear goggles if needed 4.If test tubes smash inform teacher straight away for help to clear it up. 5.Be careful with iodine do not drop on clothes as substance will stain 6. Do not squirt pipette, as there could be irritant chemicals inside that could come in contact with our eyes and skin. 7. Table should be clear and bags must be out of the way too avoid trips and falls Equipment List 1.Test tubes 2.Test tube rack 3.Cavity Tiles 4.Pipette 5.Iodine solution 6.Starch 7.Amylase 8.Beaker 9.Benedicts solution 11.Stop Watch or Clock Method 1.Collect equipment and set up as in diagram 2.Add starch to test tube 1cm. ...read more.

Conclusion

Although temperature does affect enzymes it can also denature them, which showed in my results table when I increased the temperature by 31 C above body temperature (68 C). I can tell this as it took a long time for the starch to be digested so the enzymes were less effective at digesting the starch as they had changed their shape and structure. Evaluation I could repeat the experiment several more times to get more accurate results. Also use a wider variety of temperatures as that may create a better pattern and will make the graph results improve, as there will be a wider variety and range. I would also improve by keeping the apparatus cleaner. You could change the factors that affect enzymes and see what affect that will make. I would not make too many changes as my results were reliable and my experiment was successful. ...read more.

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