• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The factors that affect the rate of an enzyme, catalysed reaction.

Extracts from this document...

Introduction

William Pearson To investigate the factors that affect the rate of an enzyme, catalysed reaction. Aim: We have been set the task of investigating factors that affect the rate of an enzyme, catalysed reaction. Background Knowledge The enzyme we are going to use is amylase. This is the first enzyme that food meets as it enters the body, it is found in the mouth. It breaks down starch, found in most carbohydrates, into glucose, a sugary substance. This is the main reaction that takes place during chemical digestion. It is necessary as the larger insoluble molecules need to be broken down into smaller soluble molecules. These are then small enough to pass through the small intestine wall into the blood stream and be carried around the body. Enzymes have 5 important properties: 1. They are always proteins, this is another reason why we have proteins in our diet. 2. They are specific in their action, this means each enzyme is specific to one job. ...read more.

Middle

Plan I am going to conduct the experiments in as controlled environment as I can. I am going to change the temperature in my experiment and the lowest temperature will be 20 C and the highest will be 70 C. To make the experiments fair I must make sure I take the samples at the same time for each experiment. I will also use the same volume of each substance each time, I will use 1cm of amylase and 2cm of starch. To get a good set of results I am going to test the substance every 10 seconds. At this time I will use a pipette to take a sample and drop it into iodine solution, while the reaction takes place the solution will turn blue/black and when it has fully reacted it will stay colourless, this is because all the starch has been turned into glucose and the iodine only reacts with starch. ...read more.

Conclusion

Evaluation The way I have conducted this experiment is to the best of my ability and the standard of the equipment I used. As I look back I can think of many floors in the way we carried the experiment out. 1. The water baths were not accurate to the correct temperature so could have affected the result. 2. We had to use our own judgement to decipher the colour of the solution. 3. The intervals between each sample were too far apart to get accurate results. 4. We were only able to carry out the experiment once for each temperature; if we did it 3 or more times it can eliminate anomalous results. 5. In some circumstances the amylase and starch had been mixed accidentally contaminating the sample making the reaction start as they were warmed up. This could make the results unreliable. If I was to carry out the experiment again I would use a higher standard of equipment and use a different method of carrying out the experiments to give a more accurate and reliable set of results. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. 'Investigating how temperature affects the rate action of the amylase enzyme on starch.'

    * 2.) I will then gather all equipment necessary for the experiment. * 3.) I will measure out 5ml of starch solution using a measuring cylinder and place into a test tube in a test tube rack. (The measuring will be done at eye level throughout each of the three tests to ensure a fair test)

  2. Trypsin. Hypothesis: - I hypothesize that as the temperature increases the rate of enzyme ...

    As the temperature of a system is increased more molecules per unit time will reach the activation energy. Thus the rate of reaction may increase. The number of collisions per unit of time will increase. In order to convert substrate into product, enzyme must collide with and bind to the substrate at the active site.

  1. Factors That Affect the Rate of an Enzyme Reaction.

    disrupts the bands which hold the active site together, thus as with high temperature the active site changes or looses it shape. As a consequence the enzymes specific substrate can no longer fit into the enzymes active site and the reaction does not take place.

  2. Investigation in to factors affecting the rate of an enzyme catalysed reaction.

    optimum pH as it works in the stomach which has an acidic pH. If the pH falls too far above or below the optimum then this also denatures the enzyme. Enzyme concentration is another factor affecting the reaction rate, as the more enzyme present the more active sites will be available for the substrate to slot into.

  1. How environmental factors affect the rate of an enzyme catalysed reaction.

    This whole procedure was repeated three times. Safety precautions:- For safety during the experiment safety glasses will be worn for the protection of the eyes. Long hair will be tied back as to keep it from getting in the solutions or covering the eyes.

  2. I aim to investigate the enzyme Tripsin, and the factors that affect the rate ...

    Some chemicals could affect the enzyme activity2. This could be positively, because they form part of the active site and so have to be present for the enzyme to work, these are called cofactors. Or negatively, because they either compete with the substrate, so making enzyme-substrate bonding less likely, or

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work