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The History, development and use of the light and electron microscope

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The History, development and use of the light and electron              


Through this assignment I will cover a range of points concerning the development of the microscope through history. My research will be based on all aspects of the microscope and the changes that took place in order for further improvement.

Anthony Van Leewenhoek, a Dutch draper and scientist, one of the pioneers of microscopy who in the late 17th century became the first man to make and use a real microscope. He made his own simple microscopes, which had a single lens and were hand held. Microscopes of that time were very lucky to achieve x50 magnification. Anthony Van Leevwenhoek developed ways to make superior lenses, by grinding and polishing a small glass ball into a lens, with a magnification of x 270. He used these lenses to make the world’s first practical microscope.

The function of any microscope is to enhance resolution. Microscope is used simply

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Various microscopists and further research lead to the development of the light microscope solved these problems.

Electron microscopes function exactly as the optical microscope except that they use a focused beam of electrons instead of light to ‘ image ‘ the specimen and gain information as to its structure and components.  Electron microscopes (EM) were developed due to the limitations of light microscopes, which are limited, by magnification and resolution. In the early 1930s there was a desire to see the fine detail of the interior structure of organic cells. This required 10, 000 x plus magnification which was just not possible using light microscopes.

Electron microscopes are scientific instrument that use a beam of highly energetic electrons to examine objects on a very fine scale. The EM has several features in providing us with a greater understanding of cellular structure;


                    The surface features of n object or ‘ how it looks ‘, the texture, direct relation between these features and materials properties (hardness, reflectivity..Etc)


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The transmission Electron Microscope (TEM) was the first type of electron microscope to be developed and is similar to the light microscope, except that it focuses beam of electrons instead of light to ‘ see through ‘ the specimen, where as the beam of electrons are absorbed by the scanning electro microscope resulting in a 3d image.

Both SEM and Tem differ in the feature they pose. ;

From the diagrams and features it can be seen that both SEM and TEM differ in the way they interpret images to the human eye, it is because of this difference that allows us to view specimens with in a range of ways..

The research I have included through this assignment has increased my in depth understanding of microscopes over all, I enjoyed researching all aspects of the project and I’m sure ill find it will enhance my knowledge of microscopes in future . .

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