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The objective of this experiment is to determine the effect of temperature to the activity of enzyme.

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Introduction

Biology TAS Experimental Report 1 Aim: The objective of this experiment is to determine the effect of temperature to the activity of enzyme. Introduction: Enzyme is indeed a globular protein, which is constructed in a 3-dimentional shape held by various bonds such as hydrogen bond. The active size of enzyme is a small part formed by several amino acids which are stabilized by the chemical bonds. Each enzyme has a unique 3-dimentional shape of active size for different metabolism. It is responsible to hold a substrate and form an enzyme-substrate complex, which would then give out the product for living cells. With the increase of temperature, the activity of enzyme also increases because the movement of enzyme is greater and more easily to collide with the substrate to form product. Thus the rate of metabolism would increase. However, if the shape of the active size is altered, the original function of the enzyme will lose, which is also known as denaturation. There are several ways for enzymes to be denatured, one of them is temperature. Since the bonds for holding the 3-dimentional shape are weak, they could easily be broken down by heat. In this experiment, we use amylase from the green beans to convert starch into maltose. The experiment is divided into two parts: the first part is the initial measurement of the enzyme activity, which aims to ensure the normal activity of the amylase; the second part is to mix the starch solution, acetate buffer and the amylase extract together in water baths with different temperature to investigate the effect of temperature on enzyme activities. ...read more.

Middle

N.A. 0 0.00208 (cor. to 3 sig. fig.) 0 0 From the data, firstly we know the amylase used in the experiment could function properly as it could convert starch into maltose. Next, it was found that only the mixtures in 40? by other group and 60? by our group could take the time reading. For the mixtures in other temperature, the time taken was very large or not recorded as the lessons were over before they could start recording. Discussions: It is the first Biology report done in F.6. As we did not have much experience to handle the experiment and missed some procedures, a lot of errors are resulted in the experiment done by both our group and other classmates and thus the data collected by us is very inaccurate compared to that learned from the biological theory. In the experiment, some assumptions were made. First, the starch in the solution would not decompose to form glucose during the experiment. Next, all the solutions in water bath should attain to the temperature same as the bath. Also, the pH value of all the solutions should be the same. Finally, the substances provided by the laboratory were correct and not be contaminated. Let's talk about the errors done in the experiment. In our group, there was no obvious error in the 1st part, but were in the 2nd part of the experiment. ...read more.

Conclusion

Another reason is that the amylase extract used by our group was double to the other group. It might be the main cause why our data was different from it. Since we used a larger amount of the amylase extract and the temperature of the water bath was not very high actually (60?), the heat given to the extract might not be enough and thus it did not attain to the temperature of 60? in 5 minutes time. In this way, some of the amylase might not be denatured and still carry the function. Moreover and the most importantly, as the volume of amylase extract used was much greater, the amount of amylase used was greater, too. This could cause a higher probability of the existence of normal amylase in the experiment. From our group, the rate of decomposition of starch is the highest among the data from different groups. If the problems are solved, the data would be much more accurate. Conclusions: The results of the experiment find out that only the solution mixtures in 40? and 60?. There are lots of mistakes in taking the experiment. For example, different methods were used in different groups and the overuse of amylase extract. That is why the data collected is not accurate enough with the biological theory. To have improvement, the instruction of the experiment should be read carefully for better preparation to the experiment next time. Reference: Biological Science 3rd edition by CAMBRIDGE Wikipedia (http://en.wikipedia.org/wiki/Main_Page) ?? ?? ?? ?? ...read more.

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