The purpose of the activity was to make standard 1% solution of glucose in water, and to use this as a control solution.

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Introduction

Standard solutions are important so that we have a known standard to compare things. The purpose of the activity was to make standard 1% solution of glucose in water, and to use this as a control solution. This particular solution of glucose was going to be made using 1g of glucose and 100ml of water thus making the percentage of glucose 1%.

Materials

  • Bunsen burner
  • Test tube
  • Squeezy bottle
  • Glucose crystals
  • Measuring boat
  • 100ml conical flask
  • Benedict’s solution
  • Tripod
  • Beaker
  • Top pan balance (an electronic scale used to measure very light weights)

Method

After collecting all the apparatus, I was ready to conduct my experiment.

First of all, I was to place the weighing boat on the top pan balance and set the balance 0. Next I was to place the glucose crystals onto the top pan balance. By carefully adding the glucose crystals, I eventually managed to get the weight of exactly 1g. The glucose from the weighing boat was then transferred to a conical flask, and the glucose that remained in the weighing boat was rinsed into the flask. This was also done for any glucose on the sides of the flask. Water was then added until there was nearly 100ml. I wanted 100ml but pouring the water from a top will not be precise; either there will be too much or too little water.

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We poured the remaining amount of water needed using the squeezy bottle as it can be poured little by little. Now that my 1% solution of glucose was made, I was ready to test my solution for glucose, and to also test for solutions ‘A’ and ‘B’ for glucose. About 4cm3 was poured into a test tube and placed into a beaker full of water. When heated, the beaker with the water will act as a water bath for the test tube. To heat the beaker, placed it on a tripod and placed it above a Bunsen burner.

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