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The reaction Between Gelatine and Trypsin.

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Biology Coursework The reaction Between Gelatine and Trypsin Aim I am going to investigate how photographic film can be recycled to be reused again. The photographic film has a silver compound on it which needs to be separated from the plastic. If the silver compound was to come off then the plastic would go clear. If we were to use an industrial process it would be too expensive, so our task is to make it cost efficient. Exposed photographic film contains black grains of silver compound. The particles of silver are attached to the film by an insoluble compound called gelatine. To achieve this goal I have been asked to find the optimum temperature at which this process is most efficient. I will use an enzyme called Trypsin to dissolve the gelatine in the photographic film. However gelatine is in soluble in water if the water is not at a high temperature. When the enzyme digests the gelatine it forms soluble amino acids. These acids dissolve and take the silver compounds with them. At the end the plastic should appear see-through. Prediction There are many important factors that will affect my experiment. The most important factors will be concentration of the Trypsin, temperature of the Trypsin and surface area of the film piece. ...read more.


For the safety precautions we used goggles as Trypsin can be dangerous if it was to go in to the eyes. This is the step by step procedure that we employed to complete the experiment: * We took a piece of the film that was pre cut to 1cm x 1cm, and placed it between pieces of cut splint. * We took 10ml3 of heated kettle water and 10ml3 of Trypsin and put them into a test tube. * We heated a kettle and poured the water into a small pan. * We put the test tube inside of the small pan and monitored the temperature of the solution inside of the test tube. * We waited until the temperature was the temperature that we desired. * We took the splint with the film and placed it in the test tube. At this point we started the stop watch. * One of our group members agitated the film so the reaction will take place faster, however this person stayed the same throughout the whole investigation as it had to be a fair test. * Another member of the group watched the film, this is because we had to find the precise moment in which the film goes clear. ...read more.


Some aspects of this experiment did prove to be difficult. For example achieving 85 degrees Celsius was difficult as it could only be achieved for a sustained time, however in this time the experiment could take place. The next time I do this experiment I will make the size of the film strips larger because if the surface area of the film was greater, the timing of the experiment will be easier. Another reason for our experiment not being so accurate, may have been the stirring of the photographic film. This could have changed our result as one piece of film could have been stirred faster than another. The trypsin molecules may have been moving faster and may have been hitting the gelatine layer much harder and more often, therefore the film would have cleared more quickly. This could have been rectified by using a mechanical stirrer, this would stir the mixture at a constant speed. If I could do another experiment linked with this I would see how much film could be economically cleared by a particular amount of trypsin. This can be used to make clearing film economically viable. I think the experiment was reliable for me to state my conclusion. As think this because there were very few anomalies. ...read more.

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