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THE TEMERATURE OF AN ALGEA BLOOM GROWTH Introduction The experiment is to determine the effect of a certain species of algae when they are growing at different temperatures. Cyanobacteria are aquatic and photosynthetic, that is, they live in the water, and can manufacture their own food. Because they are bacteria, they are quite small and usually unicellular, though they often grow in colonies large enough to see. They have the distinction of being the oldest known fossils, Cyanobacteria are still around; they are one of the largest and most important groups of bacteria on earth. They were formerly known as "blue-green algae" - but this is a misleading name! Algae are eukaryotic organisms, not prokaryotes like Cyanobacteria. Cyanobacteria are interesting for a number of reasons: They are photoautotroph, able to use CO2 as their sole carbon source and light as their energy source. However, unlike other photosynthetic bacteria, Cyanobacteria use the same photosynthetic pathway as eukaryotic cells such as algae and higher plants. Microbes are the predominant photosynthetic organisms in most aquatic environments. ...read more.


o Firstly I will need to keep the pH constant and to do that you have to use a pH7 buffer solution. o Buffer solution is a solution that ensures that the pH remains fixed at a given value, and in this case it is 7. Safety Wear goggles and a laboratory coat at all times during the experiment. Take care when handling the water bath Use the equipment safely. Aim The aim of this experiment is to find out the effect of temperature on the growth of blue-green algae and the optimum temperature that the blue-green algae grows most (best) at. Hypothesis Most bacterium grow at about 350C so therefore for this experiment since blue-green algae is a bacteria I would expect the best growth to be happening at about 350C, so therefore I will grow the algae at different temperature 350C included and see if I am right. Number of measurements The number of measurements are the number of different temperatures that I am undertaking this experiment at which are; 50C, 100C, 150C, 200C, 250C, 300C, 350C, 400C, 450C, 500C, 550C. ...read more.


o The rate increases because the enzyme and substrate molecules both have more kinetic energy so collide more often and also because more molecules have sufficient energy to overcome the (greatly reduced) activation energy. o At the optimum point it means that the maximum rate of reaction has been achieved (350C), so most of the algae will be produced at this temperature. o Then the rate decreases as more and more of the enzyme molecules denature. o The enzyme (and especially the active site) loses its shape to become a random coil, because the thermal energy breaks the hydrogen bonds holding the secondary and tertiary structure of the enzyme together. o Because of this the substrate can no longer attach to the enzyme, and the reaction is no longer catalysed. Evaluation I think that my experiment will go well from the amount of care that I have given it. To make improvement I will use smaller ranges of temperatures, so that I can be even surer that the optimum is actually 350C. ...read more.

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