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Thin layer chromatography experiment to determine which analgesic tablets contain caffeine.

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Introduction

The Experiment to determine which analgesic tablets contain caffeine. Thin layer chromatography (TLC) is a technique to determine the quantity of unknown substances in a mixture. It is based on the principle that each substance has a unique molecular weight and size which determines the distance that it travels via capillary action through a stationary phase. In the case of TLC, the stationary phase is a thin, absorbent layer, applied to a glass, metal or plastic plate. A solution of many compounds - such as prepared orange peel solution - can be separated to reveal each component using this method. http://www.ehow.com/ Equipments needed: > Thin layer chromatography plate > Large beaker > Tin foil > Micropipettes > Ruler > Pencil > notebook > two types of aspirin tablets (A and B) > safety glasses to be worn at all times > 50/50 ethanol/ethyl ethanoate solvent for dissolving sample A and B > Grinder for crushing tablet aspirin tablets A and B > Distilled water for washing and paper towels for drying > UV lamp Process Hazard risk of procedure Severity of risk Control measures and precautions Attain 1 TLC plates. ...read more.

Middle

If skin burned place effected area under cold water for few minutes. Make sure to grip the firmly with one hand and grind the the piece with the other. If fingers still end being squashed then place affected area under cold water for few minutes. Do not take the caffeine in by mouth and hands must be washed afterwards. Straightaway Spot the TLC plate with the Aspirin sample A. Label it at the top as A. Make the spot as small as possible Too much of the solution on the TLC plate has the risk of running over the TLC plate and ruin it. If too much pressure put on the hand when labeling contains the risk of piercing through the TLC plate. Low Place the TLC plate on a table and proceed with low pressure force labeling. Use a micropipette to spot the Caffeine on to the TLC plate Squash a little piece of tablet B using a grinder and add a VERY small amount of solvent (50/50 ethanol solvent for dissolving sample A). ...read more.

Conclusion

Gently place the TLC plate in the beaker, touching only the edges of the plate. Inhaling the solution which is contained inside the beaker can cause headache. Dropping the TLC plate into the solution will ruin the TLC plate. Moderate Re-cover the beaker with the tin foil cap and let the plate develop. Take out the TLC plate when the line of developing solution has approximately reached the top of the plate. Mark this line with the pencil and ruler. Let the TLC plate dry. Inhaling the solution which is contained inside the beaker can cause headache. Moving the still Wet TLC plate can cause into ripping the TLC plate Moderate Re-cover the beaker with the tin foil cap after having removed the TLC plate. Dry the plate with a hairdryer on place. Expose the spots on the TLC plate by putting the plate under UV light. Gently outline each spot with a pencil. Draw a diagram of the plate in to a notebook, noting the size and location of each spot. Looking directly into the UV light can harm the eyes. Moderate Avert facing directly into the UV light. Wear protective eye wear all throughout the experiment. ...read more.

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