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This experiment design by a group of general science to test different foods for protein present, the experiment will be focus on the present of protein in different kind of food, using biuret test.

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Introduction

Introduction This experiment design by a group of general science to test different foods for protein present, the experiment will be focus on the present of protein in different kind of food, using biuret test. Protein contains carbon, hydrogen, oxygen and nitrogen. Some have phosphorous, there are 20 amino acid that make up protein 9 of them are essential for growth, repair tissues and for build up muscles, however our body can not produce them we have to consume them. Not all protein containing foods have all 9 essential proteins. The protein of cereals, most beans and vegetables may contain all the essential amino acids. same of them we have to mixes them with other proteins. Hypothesis The following foods will contain protein such as meat, milk, fish, eggs, nuts and tuna and the following foods will not contain high amount of protein such as tomato, potatoes, bread, chocolate and rice. Animal food can contain a higher percentage of protein than vegetable food, but it also has a high percentage of fat. Material * 5 test tubes * Test tube rack * Distilled water * Chocolate (3 gram) * Tuna ( 3 gram) * Biscuit ( 3 gram) lamb meat ( 3 gram) and tomato ( 5 gram) ...read more.

Middle

through a sample and the machine determines the percent that the light is absorbed by the sample. Optical method this is measurement of protein concentration relies on optical properties, virtually all proteins show UV absorbance maximum near 280 nm. This characteristic absorbance is due almost entirely to the absorbance by the aromatic ring in the R-groups of the amino acids tryptophan and tyrosine, during this test samples are placed directly in a spectrophotometer capable of generating and measuring UV light, no chemical reagent is involved here, as in the Biuret test, because the UV absorbance is an basic property of the protein itself. the strength of the method used by industry is more reliable as the product will be supply to public, scientists analyses the method several time before the product export or supply to the public. The weakness is the equipment used in industry to test protein is expensive, therefore The experiment design by student maybe affected by those industry method because, in industry there are more equipment which measure the quantitative, error and purity whereas in college the equipment is limited. Task 2 .1(P2.P3) B The following data shows the result of the experiment performed to investigate the present of protein in different foods. Amount of foods per gram * Nat 3 gram * Kit kat chocolate 3 gram * White bread 3 gram * Rice ...read more.

Conclusion

Therefore to get reliable and accurate result on this experiment it is important to have proper facility and equipment, for instance instead of only just testing the colure if there was a test that can determine the quantity of the protein would make a big different to the experiment result. In addition an experiment starts and finishes with the factors that change during the experiment. These are the variables; purposely changing one of the variables will make a different to the experiment, for example if the experiment designer changes the amount of chemical and see the outcome, the independent variable would be the amount of the chemical and the dependent variable would be the result. Task 3.1 (P4) In conclusion, the result of the investigation shows that same food such as rice and nut they contain protein. Whereas bread and chocolate contain only sugar the test proved that by changing the colour of the food. Having doing the test the result shows that minced meat was absent of protein. Although it known as red meat contain high amount of protein, it estates this in the discussion part of the assignment, this could be error with the method or the quality of food. this experiment constantly the result of the experiment can be affected by the amount of food http://hkpho.phys.ust.hk/Training/LectNotes/experiment.pdf ?? ?? ?? ?? ...read more.

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